Sesuvium portulacastrum

  • 文章类型: Journal Article
    SesuviumPortulacastrumL.是冰植物科中的多汁盐生植物。在热带和亚热带地区的沙质海岸线和咸沼泽地中分布着各种生态型,其通用名称为海齿蛇。这些植物耐盐,干旱,和洪水应力,并已用于稳定沙丘和恢复沿海地区。随着农业土壤盐渍化程度的提高和环境中有毒金属的广泛污染,以及水体中过量的营养物质,已探索了S.Portulacastrum用于盐渍土壤的脱盐以及污染土壤中的金属以及富营养化水中的氮和磷的植物修复。此外,海胺具有营养和药用价值。组织分析表明,许多生态型富含碳水化合物,蛋白质,维生素,和矿物质营养素。佛罗里达的美洲原住民生吃它,腌制,或煮熟。在菲律宾,腌制后被称为阿恰拉。美国马齿轮轴含有高水平的蜕皮类固醇,具有抗糖尿病药,抗癌,和哺乳动物的抗炎活性。在这篇评论文章中,我们提供植物学信息,海马齿轮轴对不同胁迫的耐受性的生理和分子机制,它的营养和药用价值,及其在盐渍土壤和水体中的繁殖和生产方法。它对各种压力环境的适应性及其在生产有价值的生物活性化合物中的作用表明,马齿轮轴可作为叶类蔬菜在盐渍土壤中生产,是一种有价值的遗传资源,可用于土壤盐分和富营养化水的生物修复。
    Sesuvium portulacastrum L. is a flowering succulent halophyte in the ice plant family Aizoaceae. There are various ecotypes distributed in sandy coastlines and salty marshlands in tropical and subtropical regions with the common name of sea purslane. These plants are tolerant to salt, drought, and flooding stresses and have been used for the stabilization of sand dunes and the restoration of coastal areas. With the increased salinization of agricultural soils and the widespread pollution of toxic metals in the environment, as well as excessive nutrients in waterbodies, S. portulacastrum has been explored for the desalination of saline soils and the phytoremediation of metals from contaminated soils and nitrogen and phosphorus from eutrophic water. In addition, sea purslane has nutraceutical and pharmaceutical value. Tissue analysis indicates that many ecotypes are rich in carbohydrates, proteins, vitamins, and mineral nutrients. Native Americans in Florida eat it raw, pickled, or cooked. In the Philippines, it is known as atchara after being pickled. S. portulacastrum contains high levels of ecdysteroids, which possess antidiabetic, anticancer, and anti-inflammatory activities in mammals. In this review article, we present the botanical information, the physiological and molecular mechanisms underlying the tolerance of sea purslane to different stresses, its nutritional and pharmaceutical value, and the methods for its propagation and production in saline soils and waterbodies. Its adaptability to a wide range of stressful environments and its role in the production of valuable bioactive compounds suggest that S. portulacastrum can be produced in saline soils as a leafy vegetable and is a valuable genetic resource that can be used for the bioremediation of soil salinity and eutrophic water.
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  • 文章类型: Journal Article
    SesuviumMassulacastrum,具有传统用途的沿海药用植物已显示出有希望的生物活性,包括抗炎,抗氧化和抗菌性能。然而,该植物活性成分的作用机理尚未研究。本研究的目的是通过体外和计算机分析研究塞苏的抗癌活性。体外测定包括NO自由基清除活性,总酚和黄酮含量测定。通过单向ANOVA分析数据,并且p<0.05被认为是统计学上显著的。植物化学分析显示存在单宁,类固醇,萜类和酚类。马齿轮轴的抗氧化活性表现出一氧化氮自由基清除活性的剂量依赖性作用。计算机模拟分析显示与IR有更好的结合亲和力,IRS1和Akt分子证明了马齿轮轴的生物活性化合物对IRS-1/AKT信号通路的作用。
    Sesuvium portulacastrum, a coastal medicinal plant with traditional uses has shown promising biological activities including anti-inflammatory, antioxidant and antimicrobial properties. However, the mechanisms of action active ingredients of this plant have not been studied. Aim of the current study is to investigate the anticancer activity of Sesuvium portulacastrum using in vitro and in silico analysis. The in vitro assays included NO radical scavenging activity, total phenolic and flavonoid content determination. The data were analysed by one-way-ANOVA and p<0.05 was considered as statistically significant. The phytochemical analysis showed the presence of tannins, steroids, terpenoids and phenols. Antioxidant activity of S. portulacastrum showed the dose dependent effect of nitric oxide radical scavenging activity. In silico analysis showed a better binding affinity with IR, IRS1 and Akt molecules which demonstrated the action of bioactive compound of S. portulacastrum against IRS-1/AKT signalling pathway.
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  • 文章类型: Journal Article
    背景:Aizoaceae家族的Sesuvioides(Fenzl)Verdc是Cholistan沙漠的药用物种,巴基斯坦。这项研究的目的是确定菊科中Sesuvium属的基因组特征和系统发育位置。我们使用IlluminaHiSeq2500和配对末端测序来发布S.sesuvioides的完整叶绿体序列。
    结果:Sesuvioides的155,849bp长度的cp基因组序列具有36.8%的GC含量。亮氨酸密码子的密码子使用率最高(10.6%),19种的81个简单序列重复,和79个寡核苷酸重复。我们调查了来自23科和25个不同属的27种石竹目的系统发育。最大似然树表明Sesuvium为单系属,还有Tetragonia的妹妹.S.sesuvioides的比较,与SesuviumMassulacastrum,结晶中胚层,中胚花,并使用NCBI平台进行四聚四聚虫。在基因组的比较研究中,所有五个属都揭示了相当的cp基因组结构,基因数量和组成。所有五个物种都缺乏rps15基因和rpl2内含子。在与S.sesuvioides的大多数比较中,过渡替换(Ts)比颠换替换(Tv)更频繁,产生大于1的Ts/Tv比,Ka/Ks比值低于1。我们确定了十个高度多态性区域,包括rpl22,rpl32-trnL-UAG,trnD-GUC-trnY-GUA,trnE-UUC-trnT-GGU,trnK-UUU-rps16,trnM-CAU-atpE,trnH-GUG-psbA,psaJ-rpl33,rps4-trnT-UGU,和trnF-GAA-ndhJ.
    结论:未来将对更多的Sesuvioides和Aizoae物种进行测序后,将对整个S.sesuvioides叶绿体进行深入研究。菊科的叶绿体基因组保存完好,几乎没有改动,表明家庭的单系起源。本研究的高度多态性区域可用于建立现实和低成本的分子标记,以解决分类学差异,新物种鉴定,并发现菊科物种之间的进化联系。为了正确理解菊科的进化,进一步的物种需要测序。
    BACKGROUND: The Aizoaceae family\'s Sesuvium sesuvioides (Fenzl) Verdc is a medicinal species of the Cholistan desert, Pakistan. The purpose of this study was to determine the genomic features and phylogenetic position of the Sesuvium genus in the Aizoaceae family. We used the Illumina HiSeq2500 and paired-end sequencing to publish the complete chloroplast sequence of S. sesuvioides.
    RESULTS: The 155,849 bp length cp genome sequence of S. sesuvioides has a 36.8% GC content. The Leucine codon has the greatest codon use (10.6%), 81 simple sequence repetitions of 19 kinds, and 79 oligonucleotide repeats. We investigated the phylogeny of the order Caryophyllales\' 27 species from 23 families and 25 distinct genera. The maximum likelihood tree indicated Sesuvium as a monophyletic genus, and sister to Tetragonia. A comparison of S. sesuvioides, with Sesuvium portulacastrum, Mesembryanthemum crystallinum, Mesembryanthemum cordifolium, and Tetragonia tetragonoides was performed using the NCBI platform. In the comparative investigation of genomes, all five genera revealed comparable cp genome structure, gene number and composition. All five species lacked the rps15 gene and the rpl2 intron. In most comparisons with S. sesuvioides, transition substitutions (Ts) were more frequent than transversion substitutions (Tv), producing Ts/Tv ratios larger than one, and the Ka/Ks ratio was lower than one. We determined ten highly polymorphic regions, comprising rpl22, rpl32-trnL-UAG, trnD-GUC-trnY-GUA, trnE-UUC-trnT-GGU, trnK-UUU-rps16, trnM-CAU-atpE, trnH-GUG-psbA, psaJ-rpl33, rps4-trnT-UGU, and trnF-GAA-ndhJ.
    CONCLUSIONS: The whole S. sesuvioides chloroplast will be examined as a resource for in-depth taxonomic research of the genus when more Sesuvium and Aizoaceae species are sequenced in the future. The chloroplast genomes of the Aizoaceae family are well preserved, with little alterations, indicating the family\'s monophyletic origin. This study\'s highly polymorphic regions could be utilized to build realistic and low-cost molecular markers for resolving taxonomic discrepancies, new species identification, and finding evolutionary links among Aizoaceae species. To properly comprehend the evolution of the Aizoaceae family, further species need to be sequenced.
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  • 文章类型: Journal Article
    盐胁迫是影响全球作物生产力的主要制约因素。盐生植物的研究可以为改善经济上重要的作物以耐受盐胁迫以及更有效地使用盐生植物来修复盐碱环境提供有价值的信息。SesuviumMassulacastrumL.是一种广泛分布于热带和亚热带沿海地区的盐生植物,可以吸收大量的钠(Na)。本研究旨在分析马齿轮轴对盐胁迫的形态学反应,生理,蛋白质组学,和代谢组学水平,并对其耐盐性背后的机制有更好的理解。
    初始实验评估了在水培系统中马齿轮轴对不同浓度NaCl的形态响应,和随后的实验比较生理,蛋白质组学,在暴露于0.4MNaCl24小时后,作为即时盐胁迫(IS)至14天作为适应性盐胁迫(AS),马齿轮轴的代谢组学变化。通过这些分析,提出了一个工作模型来说明马齿轮轴对盐胁迫的综合反应。
    在0.4MNaCl中生长的植物在形态上与在对照处理中生长的植物相当。生理变化在控制中有所不同,IS,和AS植物基于测量的参数。蛋白质组学分析确定了共47和248差异表达的蛋白质(DEP)在叶和根,分别。KEGG分析显示,尤其是那些发生在根部的,主要与代谢途径有关。根代谢组学分析表明,有292种差异表达的代谢物(DEM)发生在IS植物中,有371种发生在AS植物中。其中,20.63%的DEM上调与酚酸代谢有关。
    基于蛋白质组学和代谢组学的综合分析,信号转导和酚酸代谢似乎对马齿轮轴菌耐受盐胁迫至关重要。具体来说,Ca2+,ABA,和JA信号协调调节马齿轮轴菌的耐盐性。胁迫最初通过Ca2+信号转导激活苯丙素生物合成途径,增加代谢产物的含量,比如针叶树。同时,应激通过ABA和JA信号转导抑制MAPK信号通路,促进Na固存到液泡中,以维持ROS稳态,并增强马齿轮轴对盐胁迫的耐受性。
    UNASSIGNED: Salt stress is a major constraint affecting crop productivity worldwide. Investigation of halophytes could provide valuable information for improving economically important crops to tolerate salt stress and for more effectively using halophytes to remediate saline environments. Sesuvium portulacastrum L. is a halophyte species widely distributed in tropical and subtropical coastal regions and can absorb a large amount of sodium (Na). This study was to analyze S. portulacastrum responses to salt stress at morphological, physiological, proteomic, and metabolomic levels and pursue a better understanding of mechanisms behind its salt tolerance.
    UNASSIGNED: The initial experiment evaluated morphological responses of S. portulacastrum to different concentrations of NaCl in a hydroponic system, and subsequent experiments compared physiological, proteomic, and metabolomic changes in S. portulacastrum after being exposed to 0.4 M NaCl for 24 h as immediate salt stress (IS) to 14 days as adaptive salt stress (AS). Through these analyses, a working model to illustrate the integrative responses of S. portulacastrum to salt stress was proposed.
    UNASSIGNED: Plants grown in 0.4 M NaCl were morphologically comparable to those grown in the control treatment. Physiological changes varied in control, IS, and AS plants based on the measured parameters. Proteomic analysis identified a total of 47 and 248 differentially expressed proteins (DEPs) in leaves and roots, respectively. KEGG analysis showed that DEPs, especially those occurring in roots, were largely related to metabolic pathways. Root metabolomic analysis showed that 292 differentially expressed metabolites (DEMs) occurred in IS plants and 371 in AS plants. Among them, 20.63% of upregulated DEMs were related to phenolic acid metabolism.
    UNASSIGNED: Based on the integrative analysis of proteomics and metabolomics, signal transduction and phenolic acid metabolism appeared to be crucial for S. portulacastrum to tolerate salt stress. Specifically, Ca2+, ABA, and JA signalings coordinately regulated salt tolerance in S. portulacastrum. The stress initially activated phenylpropanoid biosynthesis pathway through Ca2+ signal transduction and increased the content of metabolites, such as coniferin. Meanwhile, the stress inhibited MAPK signaling pathway through ABA and JA signal transduction, which promoted Na sequestration into the vacuole to maintain ROS homeostasis and enhanced S. portulacastrum tolerance to salt stress.
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  • 文章类型: Journal Article
    背景:SesuviumMosulacastrum是一种兼性盐生植物,能够在盐水环境中茁壮成长。尽管进行了分子研究以揭示其盐适应机制,关于盐反应直系同源物和microRNAs(miRNA)在该盐生植物中的作用的信息很少。这里,我们使用拟南芥基因组搜索了正交学,以鉴定盐反应直系同源物和Sesuvium的miRNA靶标。
    方法:直系同源物的相对倍数变化,保守的miRNA,在100mM(LS)和250mMNaCl(HS)处理下,在24h使用qRT-PCR分析Sesuvium的miRNA靶标。使用Sesuvium直向同源物和拟南芥直向同源物的表达之间的比较(拟南芥eFP浏览器数据库)来鉴定差异表达的基因。
    结果:盐处理后,我们发现Sesuvium根中的SpCIPK3(LS为1.95倍,HS为2.90倍),和SpNHX7(LS为1.61倍,HS为6.39倍),Sesuvium叶片中的SpSTPK2(LS为2.54倍,HS为7.65倍)以盐浓度特异性方式上调。在拟南芥中,这些基因要么下调,要么没有表现出显著的变异,暗示其在Sesuvium的盐生植物性质中的意义。此外,miR394a,miR396a,和miR397a与它们的靶标-Frigida相互作用蛋白1,半胱氨酸蛋白酶超家族蛋白,和推定的漆酶,分别在不同的盐处理下。
    结论:研究表明,Sesuvium的高耐盐性与不同的转录重编程有关,因此,为了获得整体的机械洞察力,全球规模的分析是必需的。
    BACKGROUND: Sesuvium portulacastrum is a facultative halophyte capable of thriving in a saline environment. Despite molecular studies conducted to unravel its salt adaptation mechanism, there is a paucity of information on the role of salt-responsive orthologs and microRNAs (miRNAs) in this halophyte. Here, we searched the orthology to identify salt-responsive orthologs and miRNA targets of Sesuvium using the Arabidopsis genome.
    METHODS: The relative fold change of orthologs, conserved miRNAs, and miRNA targets of Sesuvium was analyzed under 100 mM (LS) and 250 mM NaCl (HS) treatment at 24 h using qRT-PCR. The comparison between the expression of Sesuvium orthologs and Arabidopsis orthologs (Arabidopsis eFP browser database) was used to identify differentially expressed genes.
    RESULTS: Upon salt treatment, we found that SpCIPK3 (1.95-fold in LS and 2.90-fold in HS) in Sesuvium roots, and SpNHX7 (1.61-fold in LS and 6.39-fold in HS) and, SpSTPK2 (2.54-fold in LS and 7.65-fold in HS) in Sesuvium leaves were upregulated in a salt concentration-specific manner. In Arabidopsis, these genes were either downregulated or did not show significant variation, implicating its significance in the halophytic nature of Sesuvium. Furthermore, miRNAs like miR394a, miR396a, and miR397a exhibited a negative correlation with their targets-Frigida interacting protein 1, Cysteine proteinases superfamily protein, and Putative laccase, respectively under different salt treatments.
    CONCLUSIONS: The study revealed that the high salt tolerance in Sesuvium is associated with distinct transcriptional reprogramming, hence, to gain holistic mechanistic insights, global-scale profiling is required.
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  • 文章类型: Journal Article
    我们进行了一项研究,以检查从中国海南岛采集的12种不同生态型的无茎无茎的生长和生理变化。这些生态型受到不同浓度(0、200、400和600mmol/L)氯化钠(NaCl)盐胁迫14天。我们还分析了与应激反应相关的代谢基因的表达。在低盐胁迫下,K地区的株高等指标(0mmol/L:45%,最高为200mmol/L:80%),节间长度(0mmol/L:0.38,200mmol/L:0.87,400mmol/L:0.25和600mmol/L:1.35),以及叶面积,相对含水量,鲜重,和干重随盐浓度的增加呈总体增加趋势。然而,随着盐浓度的增加,这些指标呈下降趋势。脯氨酸和丙二醛含量随盐浓度的增加而增加。当NaCl浓度为400mmol/L时,E地区叶片中MDA含量最高(196.23%),F(94.28%),J(170.10%),与对照组相比,K(136.08%),分别。大多数材料显示叶绿素a显着下降,叶绿素b,和总叶绿素含量与对照组相比。此外,叶绿素a与叶绿素b(Rab)的比例在不同材料之间有所不同。使用主成分分析,我们确定了三种生态型(L来自兴隆村,Dan州市;水沟坡村的B,陵水县;和海洞坊公园的J,东方市)代表高,中等,和低耐盐性水平,分别,基于上述生长和生理指标。为了进一步研究相关基因在转录水平上的表达变化,我们采用qRT-PCR。结果表明,随着盐浓度的升高,SpP5CS1、SpLOX1和SpLOX1基因的相对表达量增加,这对应于脯氨酸和丙二醛含量的积累,分别。然而,SpCHL1a和SpCHL1b的相对表达模式不一致.这项研究有助于我们了解真正的盐生植物S.hypulacastrum的耐盐机制,为该领域的进一步研究提供了坚实的理论基础。
    We conducted a study to examine the growth and physiological changes in 12 different ecotypes of Sesuvium portulacastrum collected from Hainan Island in China. These ecotypes were subjected to different concentrations (0, 200, 400, and 600 mmol/L) of sodium chloride (NaCl) salt stress for 14 days. We also analyzed the expression of metabolic genes related to stress response. Under low salt stress, indicators such as plant height in region K (0 mmol/L: 45% and highest at 200 mmol/L: 80%), internode length (0 mmol/L: 0.38, 200 mmol/L: 0.87, 400 mmol/L: 0.25, and 600 mmol/L: 1.35), as well as leaf area, relative water content, fresh weight, and dry weight exhibited an overall increasing trend with the increase in salt concentration. However, as the salt concentration increased, these indicators showed a decreasing trend. Proline and malondialdehyde contents increased with higher salt concentrations. When the NaCl concentration was 400 mmol/L, MDA content in the leaves was highest in the regions E (196.23%), F (94.28%), J (170.10%), and K (136.08%) as compared to the control group, respectively. Most materials demonstrated a significant decrease in chlorophyll a, chlorophyll b, and total chlorophyll content compared to the control group. Furthermore, the ratio of chlorophyll a to chlorophyll b (Rab) varied among different materials. Using principal component analysis, we identified three ecotypes (L from Xinglong Village, Danzhou City; B from Shuigoupo Village, Lingshui County; and J from Haidongfang Park, Dongfang City) that represented high, medium, and low salt tolerance levels, respectively, based on the above growth and physiological indexes. To further investigate the expression changes of related genes at the transcriptional level, we employed qRT-PCR. The results showed that the relative expression of SpP5CS1, SpLOX1, and SpLOX1 genes increased with higher salt concentrations, which corresponded to the accumulation of proline and malondialdehyde content, respectively. However, the relative expression of SpCHL1a and SpCHL1b did not exhibit a consistent pattern. This study contributes to our understanding of the salt tolerance mechanism in the true halophyte S. portulacastrum, providing a solid theoretical foundation for further research in this field.
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  • 文章类型: Journal Article
    SesuviumMassulacastrum是一种典型的盐生植物。然而,很少有研究探讨其耐盐的分子机制。在这项研究中,代谢组,转录组,并进行了多通量全长测序分析,以研究盐度下马齿轮轴样品的显着差异代谢物(SDMs)和差异表达基因(DEGs)。已开发出完整长度的马齿轮轴转录组,其中包含39,659个非冗余的单基因。RNA-seq结果表明,参与木质素生物合成的52个DEGs可能是马齿轮轴耐盐性的原因。此外,确定了130个SDM,盐反应可归因于木质素生物合成中富含对香豆醇。在比较不同的盐处理过程后构建的共表达网络表明,对香豆醇与30个DEG相关。在这里,8个结构基因,即,Sp4CL,SpCAD,SpCCR,SpCOMT,SpF5H,SpCYP73A,SpCCoAOMT,和SpC3\'H被确定为调节木质素生物合成的重要因素。进一步的研究表明,64个推定的转录因子(TF)可能与上述基因的启动子相互作用。一起,数据揭示了一个包含重要基因的潜在调控网络,推定的TFs,和参与盐胁迫下马齿轮轴根木质素生物合成的代谢物,可作为选育优良耐盐植物的丰富有用遗传资源。
    Sesuvium portulacastrum is a typical halophyte. However, few studies have investigated its salt-tolerant molecular mechanism. In this study, metabolome, transcriptome, and multi-flux full-length sequencing analysis were conducted to investigate the significantly different metabolites (SDMs) and differentially expressed genes (DEGs) of S. portulacastrum samples under salinity. The complete-length transcriptome of S. portulacastrum was developed, which contained 39,659 non-redundant unigenes. RNA-seq results showed that 52 DEGs involved in lignin biosynthesis may be responsible for S. portulacastrum salt tolerance. Furthermore, 130 SDMs were identified, and the salt response could be attributed to the p-coumaryl alcohol-rich in lignin biosynthesis. The co-expression network that was constructed after comparing the different salt treatment processes showed that the p-Coumaryl alcohol was linked to 30 DEGs. Herein, 8 structures genes, i.e., Sp4CL, SpCAD, SpCCR, SpCOMT, SpF5H, SpCYP73A, SpCCoAOMT, and SpC3\'H were identified as significant factors in regulating lignin biosynthesis. Further investigation revealed that 64 putative transcription factors (TFs) may interact with the promoters of the above-mentioned genes. Together, the data revealed a potential regulatory network comprising important genes, putative TFs, and metabolites involved in the lignin biosynthesis of S. portulacastrum roots under salt stress, which could serve as a rich useful genetic resource for breeding excellent salt-tolerant plants.
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  • 文章类型: Journal Article
    SesuviumMassulacastrum具有很强的耐盐性,可以在盐碱沿海和内陆栖息地生长。本研究通过分析植物激素和抗氧化活性的变化,研究了马齿轮轴对高盐度的生理和分子响应。包括它们在相似的高盐度条件下的差异表达基因(DEGs)。高盐度显著影响马齿轮轴幼苗中的脯氨酸(Pro)和过氧化氢(H2O2),Pro和H2O2含量分别增加290.56和83.36%,分别,与对照相比。抗氧化活性,包括超氧化物歧化酶(SOD),过氧化物酶(POD),和过氧化氢酶(CAT),大幅增长83.05、205.14和751.87%,分别,在高盐度下。同时,脱落酸(ABA)和赤霉酸(GA3)含量与高盐处理呈相反趋势。从头转录组分析表明,36,676个单基因是匹配的,3,622个盐胁迫诱导的DEGs被鉴定为与抗氧化活性和植物激素的代谢和生物调节过程有关。在高盐度条件下,POD和SOD上调。此外,涉及生长素(SAURs和GH3)的基因的转录水平,乙烯(ERF1、ERF3、ERF114和ABR1),ABA(PP2C),和GA3(PIF3)的转运或信号被改变。本研究确定了海参高耐盐的关键代谢和生物学过程以及推定的相关基因,对于发现新的耐盐基因以促进沿海链的生态恢复具有重要意义。
    Sesuvium portulacastrum has a strong salt tolerance and can grow in saline and alkaline coastal and inland habitats. This study investigated the physiological and molecular responses of S. portulacastrum to high salinity by analyzing the changes in plant phytohormones and antioxidant activity, including their differentially expressed genes (DEGs) under similar high-salinity conditions. High salinity significantly affected proline (Pro) and hydrogen peroxide (H2O2) in S. portulacastrum seedlings, increasing Pro and H2O2 contents by 290.56 and 83.36%, respectively, compared to the control. Antioxidant activities, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), significantly increased by 83.05, 205.14, and 751.87%, respectively, under high salinity. Meanwhile, abscisic acid (ABA) and gibberellic acid (GA3) contents showed the reverse trend of high salt treatment. De novo transcriptome analysis showed that 36,676 unigenes were matched, and 3,622 salt stress-induced DEGs were identified as being associated with the metabolic and biological regulation processes of antioxidant activity and plant phytohormones. POD and SOD were upregulated under high-salinity conditions. In addition, the transcription levels of genes involved in auxin (SAURs and GH3), ethylene (ERF1, ERF3, ERF114, and ABR1), ABA (PP2C), and GA3 (PIF3) transport or signaling were altered. This study identified key metabolic and biological processes and putative genes involved in the high salt tolerance of S. portulacastrum and it is of great significance for identifying new salt-tolerant genes to promote ecological restoration of the coastal strand.
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  • 文章类型: Journal Article
    土壤盐分是严重影响植物生长和作物生产力的重要环境问题。植物修复是一种具有成本效益的解决方案,可降低土壤盐分并可能将土壤转化为作物生产。SesuviumMassulacastrum是一种典型的盐生植物,可以在高盐浓度下生长。为了探讨马齿本的耐盐机制,生根的插条在含有½Hoagland溶液的水培培养物中生长,添加或不添加400mMNa21天。在Na处理后1小时和21天采集根和叶样品,和RNA-Seq用于分析Na处理和对照植物的根和叶的转录差异。在盐胁迫下生长的植物的根和叶中鉴定出大量差异表达的基因(DEGs)。通过KEGG分析确定了与耐盐性相关的几个关键途径。结合生理数据和表达分析,似乎环核苷酸门控通道(CNGC)与Na摄取有关,而Na/H交换剂(NHX)负责Na的挤出和螯合,在盐胁迫下,促进了马齿香中Na和K之间的平衡。可溶性糖和脯氨酸被确定为盐胁迫的马齿轮轴植物中的重要渗透保护剂。谷胱甘肽代谢在清除活性氧中起重要作用。这项研究的结果表明,作为盐生植物物种的马齿轮轴具有一系列积累和耐受高水平Na的机制;因此,它可能是一种有价值的植物物种,用于盐渍土壤的植物修复。
    Soil salinity is an important environmental problem that seriously affects plant growth and crop productivity. Phytoremediation is a cost-effective solution for reducing soil salinity and potentially converting the soils for crop production. Sesuvium portulacastrum is a typical halophyte which can grow at high salt concentrations. In order to explore the salt tolerance mechanism of S. portulacastrum, rooted cuttings were grown in a hydroponic culture containing ½ Hoagland solution with or without addition of 400 mM Na for 21 days. Root and leaf samples were taken 1 h and 21 days after Na treatment, and RNA-Seq was used to analyze transcript differences in roots and leaves of the Na-treated and control plants. A large number of differentially expressed genes (DEGs) were identified in the roots and leaves of plants grown under salt stress. Several key pathways related to salt tolerance were identified through KEGG analysis. Combined with physiological data and expression analysis, it appeared that cyclic nucleotide gated channels (CNGCs) were implicated in Na uptake and Na+/H+ exchangers (NHXs) were responsible for the extrusion and sequestration of Na, which facilitated a balance between Na+ and K+ in S. portulacastrum under salt stress. Soluble sugar and proline were identified as important osmoprotectant in salt-stressed S. portulacastrum plants. Glutathione metabolism played an important role in scavenging reactive oxygen species. Results from this study show that S. portulacastrum as a halophytic species possesses a suite of mechanisms for accumulating and tolerating a high level of Na; thus, it could be a valuable plant species used for phytoremediation of saline soils.
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  • 文章类型: Journal Article
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