METHODS: The relative fold change of orthologs, conserved miRNAs, and miRNA targets of Sesuvium was analyzed under 100 mM (LS) and 250 mM NaCl (HS) treatment at 24 h using qRT-PCR. The comparison between the expression of Sesuvium orthologs and Arabidopsis orthologs (Arabidopsis eFP browser database) was used to identify differentially expressed genes.
RESULTS: Upon salt treatment, we found that SpCIPK3 (1.95-fold in LS and 2.90-fold in HS) in Sesuvium roots, and SpNHX7 (1.61-fold in LS and 6.39-fold in HS) and, SpSTPK2 (2.54-fold in LS and 7.65-fold in HS) in Sesuvium leaves were upregulated in a salt concentration-specific manner. In Arabidopsis, these genes were either downregulated or did not show significant variation, implicating its significance in the halophytic nature of Sesuvium. Furthermore, miRNAs like miR394a, miR396a, and miR397a exhibited a negative correlation with their targets-Frigida interacting protein 1, Cysteine proteinases superfamily protein, and Putative laccase, respectively under different salt treatments.
CONCLUSIONS: The study revealed that the high salt tolerance in Sesuvium is associated with distinct transcriptional reprogramming, hence, to gain holistic mechanistic insights, global-scale profiling is required.
方法:直系同源物的相对倍数变化,保守的miRNA,在100mM(LS)和250mMNaCl(HS)处理下,在24h使用qRT-PCR分析Sesuvium的miRNA靶标。使用Sesuvium直向同源物和拟南芥直向同源物的表达之间的比较(拟南芥eFP浏览器数据库)来鉴定差异表达的基因。
结果:盐处理后,我们发现Sesuvium根中的SpCIPK3(LS为1.95倍,HS为2.90倍),和SpNHX7(LS为1.61倍,HS为6.39倍),Sesuvium叶片中的SpSTPK2(LS为2.54倍,HS为7.65倍)以盐浓度特异性方式上调。在拟南芥中,这些基因要么下调,要么没有表现出显著的变异,暗示其在Sesuvium的盐生植物性质中的意义。此外,miR394a,miR396a,和miR397a与它们的靶标-Frigida相互作用蛋白1,半胱氨酸蛋白酶超家族蛋白,和推定的漆酶,分别在不同的盐处理下。
结论:研究表明,Sesuvium的高耐盐性与不同的转录重编程有关,因此,为了获得整体的机械洞察力,全球规模的分析是必需的。