Abstract：
Sesuvium portulacastrum is a typical halophyte. However, few studies have investigated its salt-tolerant molecular mechanism. In this study, metabolome, transcriptome, and multi-flux full-length sequencing analysis were conducted to investigate the significantly different metabolites (SDMs) and differentially expressed genes (DEGs) of S. portulacastrum samples under salinity. The complete-length transcriptome of S. portulacastrum was developed, which contained 39,659 non-redundant unigenes. RNA-seq results showed that 52 DEGs involved in lignin biosynthesis may be responsible for S. portulacastrum salt tolerance. Furthermore, 130 SDMs were identified, and the salt response could be attributed to the p-coumaryl alcohol-rich in lignin biosynthesis. The co-expression network that was constructed after comparing the different salt treatment processes showed that the p-Coumaryl alcohol was linked to 30 DEGs. Herein, 8 structures genes, i.e., Sp4CL, SpCAD, SpCCR, SpCOMT, SpF5H, SpCYP73A, SpCCoAOMT, and SpC3\'H were identified as significant factors in regulating lignin biosynthesis. Further investigation revealed that 64 putative transcription factors (TFs) may interact with the promoters of the above-mentioned genes. Together, the data revealed a potential regulatory network comprising important genes, putative TFs, and metabolites involved in the lignin biosynthesis of S. portulacastrum roots under salt stress, which could serve as a rich useful genetic resource for breeding excellent salt-tolerant plants.
摘要：
SesuviumMassulacastrum是一种典型的盐生植物。然而,很少有研究探讨其耐盐的分子机制。在这项研究中,代谢组，转录组，并进行了多通量全长测序分析，以研究盐度下马齿轮轴样品的显着差异代谢物（SDMs）和差异表达基因（DEGs）。已开发出完整长度的马齿轮轴转录组，其中包含39,659个非冗余的单基因。RNA-seq结果表明，参与木质素生物合成的52个DEGs可能是马齿轮轴耐盐性的原因。此外，确定了130个SDM，盐反应可归因于木质素生物合成中富含对香豆醇。在比较不同的盐处理过程后构建的共表达网络表明，对香豆醇与30个DEG相关。在这里，8个结构基因，即，Sp4CL,SpCAD,SpCCR,SpCOMT,SpF5H,SpCYP73A,SpCCoAOMT,和SpC3\'H被确定为调节木质素生物合成的重要因素。进一步的研究表明，64个推定的转录因子（TF）可能与上述基因的启动子相互作用。一起,数据揭示了一个包含重要基因的潜在调控网络，推定的TFs，和参与盐胁迫下马齿轮轴根木质素生物合成的代谢物，可作为选育优良耐盐植物的丰富有用遗传资源。
