PDF(Pubmed)
Abstract：
Sesuvium portulacastrum, a coastal medicinal plant with traditional uses has shown promising biological activities including anti-inflammatory, antioxidant and antimicrobial properties. However, the mechanisms of action active ingredients of this plant have not been studied. Aim of the current study is to investigate the anticancer activity of Sesuvium portulacastrum using in vitro and in silico analysis. The in vitro assays included NO radical scavenging activity, total phenolic and flavonoid content determination. The data were analysed by one-way-ANOVA and p<0.05 was considered as statistically significant. The phytochemical analysis showed the presence of tannins, steroids, terpenoids and phenols. Antioxidant activity of S. portulacastrum showed the dose dependent effect of nitric oxide radical scavenging activity. In silico analysis showed a better binding affinity with IR, IRS1 and Akt molecules which demonstrated the action of bioactive compound of S. portulacastrum against IRS-1/AKT signalling pathway.
摘要：
SesuviumMassulacastrum,具有传统用途的沿海药用植物已显示出有希望的生物活性，包括抗炎，抗氧化和抗菌性能。然而,该植物活性成分的作用机理尚未研究。本研究的目的是通过体外和计算机分析研究塞苏的抗癌活性。体外测定包括NO自由基清除活性，总酚和黄酮含量测定。通过单向ANOVA分析数据,并且p<0.05被认为是统计学上显著的。植物化学分析显示存在单宁，类固醇,萜类和酚类。马齿轮轴的抗氧化活性表现出一氧化氮自由基清除活性的剂量依赖性作用。计算机模拟分析显示与IR有更好的结合亲和力，IRS1和Akt分子证明了马齿轮轴的生物活性化合物对IRS-1/AKT信号通路的作用。
