目的:文献中已经描述了几种显示渗透脆性测试结果的方法。我们的目的是比较评估红细胞渗透特性的新参数的实用性。即,“溶血曲线最陡部分的斜率”和最常用的参数“红细胞脆性中位数”,以评估血液样本在长期储存过程中红细胞的稳定性。
方法:从健康供体获得10份全血样品。渗透脆性试验最初是在静脉穿刺当天进行的,随后的分析在静脉穿刺后第1,2,4,7,9,11和14天进行.平均溶血百分比值用于构建溶血曲线。溶血曲线的最陡部分估计是线性的,并创建与曲线的那些部分重叠的线。计算了这些线的斜率,并给出所得的平均值。
结果:观察到红细胞脆性中值的显着增加,从静脉穿刺开始。我们比较了每天分析的平均值。与静脉穿刺当天相比,第4天观察到红细胞脆性中值的第一个显着差异(p=0.006),值分别为0.53±0.030%和0.41±0.014%。同时,与静脉穿刺当天相比,早在第2天观察到溶血曲线最陡部分的斜率值的差异(p=0.046),值分别为-966.23±233.07和-588.01±222.85。
结论:全血样品的长期储存导致渗透脆性增加并改变溶血曲线的形状。这些变化表明,推迟渗透脆性测试可能会导致诊断不准确。这些发现表明,斜率值是评估储存过程中红细胞稳定性的更准确的参数,与常用的红细胞脆性中值相比。因此,它具有潜在的重要性,可以补充OFT的实验室结果。因此,将这些结果与OFT测试的结果一起提供可能是有用的。
OBJECTIVE: Several ways for presenting the results of osmotic fragility test have been described in the literature. Our aim was to compare the utility of a novel parameter for assessment of erythrocyte osmotic properties, i.e., \'Slope of the steepest part of hemolysis curve\' with the most frequently used parameter \'Median corpuscular fragility\' in order to assess the stability of erythrocytes in a blood sample during prolonged storage.
METHODS: Ten whole blood samples were obtained from healthy donors. The osmotic fragility test was initially conducted on the day of venipuncture, and subsequent analyses were carried out on days 1, 2, 4, 7, 9, 11, and 14 after the venipuncture. Mean hemolysis percentage values were used to construct hemolysis curves. The steepest parts of hemolysis curves were estimated to be linear, and lines that overlapped those parts of the curves were created. The slope of these lines was calculated, and the resulting mean values are presented.
RESULTS: A significant increase in Median corpuscular fragility values was observed, starting from day of venipuncture. We compared the average values for each day of analysis. The first significant difference in Median corpuscular fragility values was observed on day 4 compared to the day of venipuncture (p=0.006), with values 0.53±0.030 % and 0.41±0.014% respectively. Meanwhile, differences in the values of the slopes of the steepest parts of hemolysis curves were observed as early as day 2 when compared to the day of venipuncture (p=0.046), with values of -966.23±233.07 and -588.01±222.85, respectively.
CONCLUSIONS: Prolonged storage of whole blood samples leads to an increase in osmotic fragility and alters the shape of the hemolysis curve. These changes suggest that postponing the osmotic fragility test could lead to diagnostic inaccuracies. These findings suggest that slope value is a more accurate parameter for evaluating erythrocyte stability during storage, compared to commonly used Median corpuscular fragility value. Hence, it has potential importance and can be complementary to the laboratory result of the OFT. Therefore, it can be useful to provide these results jointly with the results of the OFT test.