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Abstract：
Sea buckthorn and Japanese knotweed are known in many traditional medicine systems to be a great source of bioactive substances. This research aims to compare the bioactivity and protective effects of the phenolic extracts of leaves from sea buckthorn and roots and leaves from the Japanese knotweed on erythrocytes. The polyphenol composition of the extract was analyzed using UPLC-PDA-ESI-MS/MS. The extracts\' toxicity and impact on the erythrocytes\' osmotic fragility were measured spectrophotometrically. The antioxidant activity was determined based on the inhibition of oxidation of erythrocytes and their membrane induced by 2,2\'-Azobis(2-methylpropionamidine) dihydrochloride (AAPH),measured spectrophotometrically and using fluorimetry. To find the possible mechanism of the extracts\' action, extract-modified cells were observed under a microscope, and the potential localization of the extract\'s phytochemical composition was checked using fluorescent probes. The results showed that the used extracts are not toxic to erythrocytes, increase their osmotic resistance, and successfully protect them against free radicals. Extract components localize on the outer part of the membrane, where they can scavenge the free radicals from the environment. Altogether, the presented extracts can greatly protect living organisms against free radicals and can be used to support the treatment of diseases caused by excess free radicals.
摘要：
沙棘和日本虎杖在许多传统医学系统中被认为是生物活性物质的重要来源。这项研究旨在比较沙棘叶，根和叶的酚类提取物对红细胞的生物活性和保护作用。使用UPLC-PDA-ESI-MS/MS分析提取物的多酚组成。用分光光度法测定提取物的毒性和对红细胞渗透脆性的影响。抗氧化活性是根据2,2'-偶氮双（2-甲基丙脒）二盐酸盐（AAPH）诱导的红细胞及其膜氧化的抑制作用确定的，用分光光度法和荧光法测量。为了找到提取物作用的可能机制，在显微镜下观察提取物修饰的细胞，并使用荧光探针检查提取物的植物化学成分的潜在定位。结果表明，使用的提取物对红细胞没有毒性，增加它们的渗透阻力，并成功地保护他们免受自由基的侵害。提取成分位于膜的外部，他们可以从环境中清除自由基。总之,所提供的提取物可以极大地保护生物体免受自由基的侵害，并可用于支持治疗由过量自由基引起的疾病。
