伏马菌素B1(FB1)是自然界中一种重要的霉菌毒素,严重威胁着人类和动物的健康。但其毒性和潜在致癌性的具体靶点和分子机制尚不清楚。在这项研究中,我们首先检测了FB1对细胞活力的影响,生物物理特性,迁移能力,和人脐静脉内皮细胞(HUVECs)的活性氧(ROS)。随后,通过免疫荧光和实时PCR分析细胞骨架结构及其结合蛋白的变化,分别。结果表明,FB1可以剂量依赖性地抑制HUVECs的活力。用FB1治疗HUVECs后,低张抗性,细胞表面电荷,细胞膜流动性,迁移能力减弱,而ROS水平显著升高。此外,HUVECs的细胞骨架结构发生了显著变化,一些重要的肌动蛋白结合蛋白的mRNA表达发生了改变。因此,这项研究表明,FB1可以影响HUVECs的迁移和细胞骨架结构,这为进一步理解FB1毒性的分子机制提供了新的视角。
Fumonisin B1 (FB1) is an important mycotoxin in nature and is a serious threat to human and animal health, but its specific target and molecular mechanism of the toxicity and potential carcinogenicity remain unclear. In this study, we first detected the effects of FB1 on the cell viability, biophysical properties, migration ability, and reactive oxygen species (ROS) of human umbilical vein endothelial cells (HUVECs). Subsequently, changes in the cytoskeletal structure and its binding proteins were analyzed by immunofluorescence and real-time PCR, respectively. The results showed that FB1 could inhibit the viability of HUVECs in a dose-dependent manner. After treatment of HUVECs with FB1, the hypotonic resistance, cell surface charges, cell membrane fluidity, and migration ability were weakened, whereas the ROS levels were significantly increased. Moreover, the cytoskeletal structure of the HUVECs was significantly changed, and the mRNA expression of some important actin-binding proteins was altered. Therefore, this study revealed that FB1 can affect the migration and cytoskeletal structure of HUVECs, which provides a new perspective for further understanding the molecular mechanisms of FB1 toxicity.