太平洋鲑鱼的中枢神经系统在整个生命中保留了胚胎结构的迹象,并且在大脑的增殖区域中保留了大量的神经上皮神经干细胞(NSC),特别是。然而,对虹鳟鱼的成人神经系统和神经发生研究,Oncorhynchusmykiss,是有限的。这里,我们研究了谷氨酰胺合成酶(GS)的定位,波形蛋白(Vim),和Nestin(Nes),以及在胚胎后时期形成的神经元,标记有doublecoortin(DC),在使用免疫组织化学方法和Western免疫印迹的Oncornchusmykiss成年小脑和脑干的稳态生长条件下。我们观察到波形蛋白(Vim)的分布,nestin(Nes),和谷氨酰胺合成酶(GS),在小脑和鳟鱼脑干的胚胎型(神经上皮细胞)和成体型(放射状神经胶质)的aNSPCs中发现,具有某些特征。成体神经干/祖细胞(aNSPCs)的群体表达GS,Vim,和Nes有不同的形态,本地化,鳟鱼小脑和脑干的簇形成模式,它表示形态和,显然,这些细胞的功能异质性。PCNA的免疫标记显示虹鳟鱼的小脑和脑干中含有增殖细胞的区域与表达Vim的区域一致,Nes,和GS。双重免疫标记揭示了脑干PVZ中神经上皮型细胞中的PCNA/GSPCNA/Vim共表达模式。在脑干边缘区检测到RG中的PCNA/GS共表达。对鳟鱼小脑和脑干中DC分布的免疫组织化学研究结果表明,该标记物在各种细胞群中的高表达水平。这可能表明:(i)成年鳟鱼的小脑和脑干中成年神经元的高产量,(ii)鳟鱼小脑和脑干神经元的高可塑性。我们假设鳟鱼脑中新细胞的来源,以及PVZ和SMZ,含有增殖细胞,可能是含有PCNA阳性和沉默(PCNA阴性)的局部神经源性壁龛,但是表达NSC标记,细胞。表达DC的细胞的鉴定,Vim,并在鳟鱼的IX-X颅神经核中进行了Nes。
The central nervous system of Pacific salmon retains signs of embryonic structure throughout life and a large number of neuroepithelial neural stem cells (NSCs) in the proliferative areas of the brain, in particular. However, the adult nervous system and neurogenesis studies on rainbow trout, Oncorhynchus mykiss, are limited. Here, we studied the localization of glutamine synthetase (GS), vimentin (Vim), and nestin (Nes), as well as the neurons formed in the postembryonic period, labeled with doublecortin (DC), under conditions of homeostatic growth in adult cerebellum and brainstem of Oncorhynchus mykiss using immunohistochemical methods and Western Immunoblotting. We observed that the distribution of vimentin (Vim), nestin (Nes), and glutamine synthetase (GS), which are found in the aNSPCs of both embryonic types (neuroepithelial cells) and in the adult type (radial glia) in the cerebellum and the brainstem of trout, has certain features. Populations of the adult neural stem/progenitor cells (aNSPCs) expressing GS, Vim, and Nes have different morphologies, localizations, and patterns of cluster formation in the trout cerebellum and brainstem, which indicates the morphological and, obviously, functional heterogeneity of these cells. Immunolabeling of PCNA revealed areas in the cerebellum and brainstem of rainbow trout containing proliferating cells which coincide with areas expressing Vim, Nes, and GS. Double immunolabeling revealed the PCNA/GS PCNA/Vim coexpression patterns in the neuroepithelial-type cells in the PVZ of the brainstem. PCNA/GS coexpression in the RG was detected in the submarginal zone of the brainstem. The results of immunohistochemical study of the DC distribution in the cerebellum and brainstem of trout have showed a high level of expression of this marker in various cell populations. This may indicate: (i) high production of the adult-born neurons in the cerebellum and brainstem of adult trout, (ii) high plasticity of neurons in the cerebellum and brainstem of trout. We assume that the source of new cells in the trout brain, along with PVZ and SMZ, containing proliferating cells, may be local neurogenic niches containing the PCNA-positive and silent (PCNA-negative), but expressing NSC markers, cells. The identification of cells expressing DC, Vim, and Nes in the IX-X cranial nerve nuclei of trout was carried out.