PDF(Pubmed)
Abstract：
Astaxanthin (AST) is a natural compound derived from shellfish, microorganisms, and algae, with several healthy properties. For this reason, it is widely used in the diet of humans and animals, such as pigs, broilers, and fish, where its addition is related to its pigmenting properties. Moreover, AST\'s ability to reduce free radicals and protect cells from oxidative damage finds application during the weaning period, when piglets are exposed to several stressors. To better elucidate the mechanisms involved, here we generate ad hoc pig and rainbow trout in vitro platforms able to mimic the intestinal mucosa. The morphology is validated through histological and molecular analysis, while functional properties of the newly generated intestinal barriers, both in porcine and rainbow trout models, are demonstrated by measuring trans-epithelial electrical resistance and analyzing permeability with fluorescein isothiocyanate-dextran. Exposure to AST induced a significant upregulation of antioxidative stress markers and a reduction in the transcription of inflammation-related interleukins. Altogether, the present findings demonstrate AST\'s ability to interact with the molecular pathways controlling oxidative stress and inflammation both in the porcine and rainbow trout species and suggest AST\'s positive role in prevention and health.
摘要：
虾青素(AST)是一种来自贝类的天然化合物,微生物,和藻类，有几个健康的属性。出于这个原因,它广泛用于人类和动物的饮食，比如猪,肉鸡,鱼,其中它的添加与它的着色特性有关。此外，AST的能力，以减少自由基和保护细胞免受氧化损伤发现在断奶期间的应用，当仔猪暴露在几种压力下。为了更好地阐明其中的机制，在这里，我们产生特设猪和虹鳟鱼体外平台，能够模拟肠粘膜。形态学是通过组织学和分子分析验证，而新产生的肠道屏障的功能特性，在猪和虹鳟鱼模型中，通过测量跨上皮电阻并用异硫氰酸荧光素-葡聚糖分析渗透性来证明。暴露于AST诱导抗氧化应激标志物的显著上调和炎症相关白细胞介素转录的减少。总之,本研究结果证明了AST在猪和虹鳟鱼中与控制氧化应激和炎症的分子途径相互作用的能力，并表明AST在预防和健康中的积极作用。
