这项研究的重点是比较代谢概况和两种类固醇类型的影响:天然和合成,特别是虹鳟鱼(Oncorhynchusmykiss)中不同浓度(1.5、2和3mg/kg)的17α-甲基睾丸激素(17α-MT)。在为期75天的喂养试验中,增长指标,如饲料效率,每日特定生长,活体重增加,总体重增加,每15天系统监测生存率。在喂养试验结束时,组织病理学,免疫组织化学,和代谢组分析在高浓度组(3mg/kg天然和3mg/kg合成),其中确定了最低的存活率。关键发现表明,激素的类型显着影响生长参数。虽然一些天然类固醇增强了某些生长方面,合成变体通常会产生更好的结果。代谢组学分析强调了色氨酸代谢的显着变化,嘌呤,叶酸,初级胆汁酸,磷酸盐,次磷酸盐,和通过细胞色素P450途径的外源性物质。组织病理学,天然激素组表现出相似的睾丸,肝,肌肉,吉尔,大脑,肾,和肠道组织结构来控制,通过免疫组织化学观察到轻微的DNA损伤和细胞凋亡。相反,合成激素组表现出中度DNA损伤和组织病理学轻度变性和坏死改变.
This
study focuses on the comparative metabolic profiling and effects of two steroid types: natural and synthetic, specifically 17α-methyl testosterone (17α-MT) at varying concentrations (1.5, 2, and 3 mg/kg) in rainbow trout (Oncorhynchus mykiss). Over a 75-day feeding
trial, growth metrics, such as feed efficiency, daily specific growth, live weight gain, total weight gain, and survival rate were systematically monitored every 15 days. At the end of the feeding
trial, histopathology, immunohistochemistry, and metabolome analyses were performed in the high-concentration groups (3 mg/kg natural and 3 mg/kg synthetic), in which the lowest survival rate was determined. Key findings reveal that the type of hormone significantly influences growth parameters. While some natural steroids enhanced certain growth aspects, synthetic variants often yielded better results. The metabolomic analysis highlighted significant shifts in the metabolism of tryptophan, purine, folate, primary bile acids, phosphonates, phosphinates, and xenobiotics via cytochrome P450 pathways. Histopathologically, the natural hormone groups showed similar testicular, hepatic, muscular, gill, cerebral, renal, and intestinal tissue structures to the control, with minor DNA damage and apoptosis observed through immunohistochemistry. Conversely, the synthetic hormone groups exhibited moderate DNA damage and mild degenerative and necrotic changes in histopathology.