■生长分化因子15(GDF15)是一种在各种压力下表达的mitokine,其循环水平随年龄增长而增加,并与许多病理状况有关。包括肌肉萎缩和肌肉减少症.然而,循环GDF15(c-GDF15)作为肌肉减少症的生物标志物的应用仍存在争议.此外,GDF15细胞内前体的作用,pro-GDF15,在人骨骼肌(SM-GDF15)中尚未完全了解。为了澄清这些观点,两种形式的GDF15与肌肉力量参数的关联,身体成分,研究了代谢和炎症。
■在血浆和肌肉活检中评估c-GDF15和SM-GDF15的水平,分别,健康受试者(HS)和下肢活动障碍(LLMI)患者,年轻(<40岁)或老年(>70岁)。分析中包括的其他参数是等距四头肌强度(IQS),BMI,瘦肉和脂肪质量百分比,股外侧肌厚度,以及脂联素的循环水平,瘦素,Resistin,IGF-1,胰岛素,IL6、IL15和c-PLIN2。主成分分析(PCA),进行典型判别分析(CDA)和接收操作特性(ROC)分析。
■c-GDF15而不是SM-GDF15水平与HS和LLMI中IQS和IGF-1水平降低相关,而仅在LLMI中与抵抗素水平升高相关。此外,在LLMI中,c-GDF15和SM-GDF15水平均与IL-6水平相关,但有趣的是,SM-GDF15在LLMI中相对于HS较低。此外,PCA和CDA可以根据这些参数区分四组受试者.特别是HS,根据SM-GDF15、c-GDF15和胰岛素水平区分70岁以上或40岁以下的LLMI,分别。
■我们的数据支持c-GDF15水平可以用作肌肉质量和力量下降的生物标志物的想法。此外,提示c-GDF15相对于SM-GDF15具有不同的诊断意义,SM-GDF15可能与健康和活跃状态有关.
UNASSIGNED: Growth Differentiation Factor 15 (GDF15) is a mitokine expressed in response to various stresses whose circulating levels increase with age and are associated with numerous pathological conditions, including muscle wasting and sarcopenia. However, the use of circulating GDF15 (c-GDF15) as a biomarker of sarcopenia is still debated. Moreover, the role of GDF15 intracellular precursor, pro-GDF15, in human skeletal muscle (SM-GDF15) is not totally understood. In order to clarify these points, the association of both forms of GDF15 with parameters of muscle strength, body composition, metabolism and inflammation was investigated.
UNASSIGNED: the levels of c-GDF15 and SM-GDF15 were evaluated in plasma and muscle biopsies, respectively, of healthy subjects (HS) and patients with lower limb mobility impairment (LLMI), either young (<40 years-old) or old (>70 years-old). Other parameters included in the analysis were Isometric Quadriceps Strength (IQS), BMI, lean and fat mass percentage, Vastus lateralis thickness, as well as circulating levels of Adiponectin, Leptin, Resistin, IGF-1, Insulin, IL6, IL15 and c-PLIN2. Principal Component Analysis (PCA), Canonical Discriminant Analysis (CDA) and Receiving Operating Characteristics (ROC) analysis were performed.
UNASSIGNED: c-GDF15 but not SM-GDF15 levels resulted associated with decreased IQS and IGF-1 levels in both HS and LLMI, while only in LLMI associated with increased levels of Resistin. Moreover, in LLMI both c-GDF15 and SM-GDF15 levels were associated with IL-6 levels, but interestingly SM-GDF15 is lower in LLMI with respect to HS. Furthermore, a discrimination of the four groups of subjects based on these parameters was possible with PCA and CDA. In particular HS, LLMI over 70 years or under 40 years of age were discriminated based on SM-GDF15, c-GDF15 and Insulin levels, respectively.
UNASSIGNED: our data support the idea that c-GDF15 level could be used as a biomarker of decreased muscle mass and strength. Moreover, it is suggested that c-GDF15 has a different diagnostic significance with respect to SM-GDF15, which is likely linked to a healthy and active state.