■胃肠炎约占儿童死亡人数的10%,尤其是免疫功能低下的儿童。在伊朗,有关RNA病毒引起的胃肠道感染患病率的研究很少。该研究的目的是使用多重PCR评估引起腹泻的RNA病毒的检测。
■收集了130名患有急性淋巴细胞白血病的腹泻患儿的粪便样本,非霍奇金淋巴瘤,和视网膜母细胞瘤.RNA提取和cDNA合成后,进行多重PCR以评估轮状病毒的存在,诺如病毒,星状病毒,和肠道病毒。
■有9个(6.9%),7(5.4%),3(2.3%),和6例(4.6%)轮状病毒,诺如病毒,星状病毒,检测到肠道病毒,分别。观察到1例星形病毒和诺如病毒共同感染。
■这是伊朗的第一份报告,该报告确定了在免疫功能低下的儿童中存在引起腹泻的常见RNA病毒。提高对这些病毒的认识将使医疗保健专业人员能够改进策略和政策,以控制由这些病毒引起的传播和感染。
UNASSIGNED: Gastroenteritis accounts for about 10% of the deaths among children, especially in immunocompromised children. Few studies on the prevalence of gastrointestinal infections caused by RNA viruses have been done in Iran. The aim of the study was to evaluate the detection of RNA viruses causing diarrhoea using a multiplex PCR.
UNASSIGNED: Stool samples were collected from 130 paediatric patients with diarrhoea who had acute lymphocytic leukaemia, non-Hodgkin lymphoma, and retinoblastoma. After RNA extraction and synthesis of cDNA, multiplex PCR was done to evaluate the presence of rotavirus, norovirus, astrovirus, and enterovirus.
UNASSIGNED: There were 9 (6.9%), 7 (5.4%), 3 (2.3%), and 6 (4.6%) cases of rotavirus, norovirus, astrovirus, and enterovirus detected, respectively. One case of co-infection with astrovirus and norovirus was observed.
UNASSIGNED: This is the first report from Iran which identified the presence of common RNA viruses causing diarrhoea in immunocompromised children. Increased awareness of these viruses will enable healthcare professionals to improve strategies and policies to control spread and infection caused by these viruses.