本研究旨在阐明关键的调控分子,特别是信使RNA(mRNA),长链非编码RNA(lncRNA),和microRNAs(miRNAs)及其在脊髓损伤(SCI)发生发展中的作用。SCI的表达谱(GSE45006、GSE19890和GSE125630)来源于基因表达综合(GEO)数据库。通过比较不同时间点的SCI大鼠与没有SCI的大鼠,我们鉴定了差异表达的mRNA(DEmRNAs),lncRNAs(DElncRNAs),和miRNA(DEmiRNA)。GSE45006数据集促进了DMRNAs的生产,然后使用Mfuzz进行聚类。随后,我们构建了一个蛋白质-蛋白质相互作用(PPI)网络,并预期miRNA-mRNA和lncRNA-mRNA之间的相互作用对.这些对有助于形成涉及lncRNA-miRNA-mRNA相互作用的调节网络。此外,我们对这些基因网络中的DEmRNAs进行了功能富集研究.使用GSE45006数据集鉴定了总共2313个DMRNAs,来自GSE19890的111个DEmiRNA。从GSE125630中,我们提取了154个DElncRNAs和2322个DEmRNAs。我们的分析揭示了294个上调的DMRNAs,分组到向上集群中,和407个下调的DMRNAs,形成向下的集群。PPI网络中的关键枢纽基因,比如Rhof,Vav1,Lyz2,Rab3a,林,Cyfip1,Gns,和Nckap1l,已确定。此外,该研究成功构建了一个竞争内源性RNA(ceRNA)网络,揭示了55个独特的lncRNA-miRNA-mRNA连接对。我们的研究建立了一个与SCI相关的ceRNA网络,确定了几个关键的lncRNA-miRNA-mRNA连接对,与疾病的发病和进展密切相关。值得注意的是,重要的协会,包括AABR07041411.1-miR-125a-5p-Slc4a7和Smg1-rno-miR-331-3p-Tlr4对,被观察到在这种生物学背景下发挥重大影响。
This study aims to elucidate the key regulatory molecules, specifically messenger RNAs (mRNAs), long noncoding RNAs (lncRNAs), and microRNAs (miRNAs) and their roles in the development and progression of spinal cord injury (SCI). Expression profiles (GSE45006, GSE19890, and GSE125630) for SCI were sourced from the Gene Expression Omnibus (GEO) database. By comparing rats with SCI at various time points against those without SCI, we identified differentially expressed mRNAs (DEmRNAs), lncRNAs (DElncRNAs), and miRNAs (DEmiRNAs). The GSE45006 dataset facilitated the production of DEmRNAs, which were then clustered using Mfuzz. Subsequently, we constructed a protein-protein interaction (PPI) network and anticipated interaction pairs between miRNA-mRNA and lncRNA-mRNA. These pairs were instrumental in forming a regulatory network involving lncRNA-miRNA-mRNA interactions. Additionally, we conducted functional enrichment studies on the DEmRNAs within these gene networks. A total of 2313 DEmRNAs were identified using the GSE45006 dataset, alongside 111 DEmiRNAs from GSE19890. From GSE125630, we extracted 154 DElncRNAs and 2322 DEmRNAs. Our analysis revealed 294 up-regulated DEmRNAs, grouped into the up-cluster, and 407 down-regulated DEmRNAs, forming the down-cluster. Key hub genes in the PPI network, such as Rhof, Vav1, Lyz2, Rab3a, Lyn, Cyfip1, Gns, and Nckap1l, were identified. Additionally, the study successfully constructed a competing endogenous RNA (ceRNA) network, revealing 55 unique lncRNA-miRNA-mRNA link pairs. Our research established a ceRNA network associated with SCI, identifying several critical lncRNA-miRNA-mRNA connection pairs integral to the disease\'s onset and progression. Notably, significant associations, including the AABR07041411.1-miR-125a-5p-Slc4a7 and the Smg1-rno-miR-331-3p-Tlr4 pairs, were observed to exert a significant influence within this biological context.