作为一种低热量的糖,D-阿洛酮糖由D-阿洛酮糖3-差向异构酶(DAE)催化的D-果糖产生。这里,为了提高催化活性,稳定性,和DAE的可加工性,我们报道了一种通过形成有机-无机杂化纳米花(NF-DAEs)并将它们共固定在树脂上以形成复合材料(Re-NF-DAEs)的新方法。通过将DAE与金属离子(Co2+,Cu2+,Zn2+,Ca2+,Ni2+,Fe2+,和Fe3+)在PBS缓冲液中,并通过扫描电子显微镜(SEM)进行了分析,傅里叶变换红外光谱,和X射线衍射。所有的NF-DAEs都显示出比游离DAE更高的催化活性,具有Ni2+的NF-DAE(NF-DAE-Ni)达到最高相对活性218%。NF-DAEs提高了DAE的热稳定性,NF-DAE-Co的最长半衰期达到228分钟,而游离DAE在55°C时为105分钟。为了进一步提高NF-DAEs在实际应用中的回收性能,我们组合树脂和NF-DAEs以形成Re-NF-DAEs。树脂和NF-DAE共同影响复合材料的性能,和ReA(LXTE-606中性疏水环氧基聚丙烯大网状树脂)基复合材料(ReA-NF-DAEs)表现出优异的相对活性,热稳定性,储存稳定性,和可加工性。ReA-NF-DAEs能够重新用于催化从D-果糖到D-阿洛酮糖的转化,并在八个周期后保留了60%以上的活动。
As a low-calorie sugar, D-allulose is produced from D-fructose catalyzed by D-allulose 3-epimerase (DAE). Here, to improve the catalytic activity, stability, and processability of DAE, we reported a novel method by forming organic-inorganic hybrid nanoflowers (NF-DAEs) and co-immobilizing them on resins to form composites (Re-NF-DAEs). NF-DAEs were prepared by combining DAE with metal ions (Co2+, Cu2+, Zn2+, Ca2+, Ni2+, Fe2+, and Fe3+) in PBS buffer, and were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction. All of the NF-DAEs showed higher catalytic activities than free DAE, and the NF-DAE with Ni2+ (NF-DAE-Ni) reached the highest relative activity of 218%. The NF-DAEs improved the thermal stability of DAE, and the longest half-life reached 228 min for NF-DAE-Co compared with 105 min for the free DAE at 55 °C. To further improve the recycling performance of the NF-DAEs in practical applications, we combined resins and NF-DAEs to form Re-NF-DAEs. Resins and NF-DAEs co-effected the performance of the composites, and ReA (LXTE-606 neutral hydrophobic epoxy-based polypropylene macroreticular resins)-based composites (ReA-NF-DAEs) exhibited outstanding relative activities, thermal stabilities, storage stabilities, and processabilities. The ReA-NF-DAEs were able to be reused to catalyze the conversion from D-fructose to D-allulose, and kept more than 60% of their activities after eight cycles.