PDF(Pubmed)
Abstract：
Dopamine is one of the significant neurotransmitters and its monitoring in biological fluids is a critical issue in healthcare and modern biomedical technology. Here, we have developed a dopamine biosensor based on surface plasmon resonance (SPR). For this purpose, the carboxymethyl dextran SPR chip was used as a surface to immobilize laccase as a bioaffinity recognition element. Data analysis exhibited that the acidic pH value is the optimal condition for dopamine interaction. Calculated kinetic affinity (KD) (48,545 nM), obtained from a molecular docking study, showed strong association of dopamine with the active site of laccase. The biosensor exhibited a linearity from 0.01 to 189 μg/ml and a lower detection limit of 0.1 ng/ml (signal-to-noise ratio (S/N) = 3) that is significantly higher than the most direct dopamine detecting sensors reported so far. Experiments for specificity in the presence of compounds that can co-exist with dopamine detection such as ascorbic acid, urea and L-dopa showed no significant interference. The current dopamine biosensor with high sensitivity and specificity, represent a novel detection tool that offers a label-free, simple procedure and cost effective monitoring system.
摘要：
多巴胺是重要的神经递质之一，其在生物体液中的监测是医疗保健和现代生物医学技术中的关键问题。这里,我们开发了一种基于表面等离子体共振（SPR）的多巴胺生物传感器。为此,以羧甲基葡聚糖SPR芯片为表面固定化漆酶作为生物亲和识别元件。数据分析表明，酸性pH值是多巴胺相互作用的最佳条件。计算的动力学亲和力（KD）（48,545nM），从分子对接研究中获得，显示多巴胺与漆酶活性位点的强关联。生物传感器的线性范围为0.01至189μg/ml，检测下限为0.1ng/ml（信噪比（S/N）=3），显着高于迄今为止报道的最直接的多巴胺检测传感器。在存在可以与多巴胺检测共存的化合物（如抗坏血酸）的情况下进行特异性实验，尿素和左旋多巴无明显干扰。目前多巴胺生物传感器具有较高的灵敏度和特异性,代表一种新颖的检测工具，提供无标签，简单的程序和成本有效的监测系统。
