Abstract：
The objectives were to optimize the reaction conditions for C10:0 incorporation into grapeseed (GS) oil, characterize the structured lipid (SL) product, and study the changes in antioxidant activity of the SL. Taguchi method was used to optimize C10:0 incorporation by combining parameters in a total of 9 experiments. Lipozyme ® RM IM (Rhizomucor miehei immobilized lipase) and Lipozyme ® 435 (Candida antarctica recombinant immobilized lipase) were used as biocatalysts for the acidolysis reactions. C10:0 incorporation and triacylglycerol (TAG) species of the SL were analyzed to determine optimal conditions and enzyme type that gave higher incorporation. The optimal conditions were the same for both enzymes as follows: substrate molar ratio 1:3 (GS oil: C10:0), enzyme load 5% (w/w) of substrates, temperature 65℃, and time 12 h. HPLC analysis of SL gave MLM-type TAG species of 11.51±0.11 mol% and 12.68±0.34 mol% for Lipozyme ® RM IM and Lipozyme ® 435, respectively. GC analysis indicated that C10:0 incorporated at the sn-1,3 positions of the SL were 46.03±0.55 mol% and 47.28±1.22 mol%, respectively, for Lipozyme ® RM IM and Lipozyme ® 435. However, the total C10:0 incorporated into TAG species with Lipozyme ® RM IM was significantly higher (60.08±0.04 mol%) compared to 50.78±0.44 mol% for Lipozyme ® 435. Scaled-up (300 g) acidolysis reaction and characterization were done on SL synthesized using Lipozyme ® RM IM. SL reaction product was purified using short path distillation and fully characterized in terms of lipid classes, tocopherol, thermal behavior, and oxidative stability. The yield of purified scaled-up SL after short path distillation (SPD) was 72.96 wt%. The antioxidant in SL was reduced after SPD due to loss of tocopherols. This MLM-type-SL synthesized within 12 h using Lipozyme ® RM IM had a high content of C10:0 and may have functional and health benefits.
摘要：
目的是优化C10：0掺入葡萄籽（GS）油中的反应条件，表征结构化脂质（SL）产品，并研究了SL抗氧化活性的变化。通过在总共9个实验中组合参数,使用Taguchi方法来优化C10:0掺入。Lipozyme®RMIM（Rhizomucormiehei固定化脂肪酶）和Lipozyme®435（南极假丝酵母重组固定化脂肪酶）用作酸解反应的生物催化剂。分析SL的C10:0掺入和三酰甘油(TAG)种类以确定最佳条件和给予较高掺入的酶类型。两种酶的最佳条件如下：底物摩尔比1：3（GS油：C10：0），酶负载5%(w/w)的底物，温度65℃,SL的HPLC分析对于Lipozyme®RMIM和Lipozyme®435分别给出11.51±0.11mol%和12.68±0.34mol%的MLM型TAG种类。GC分析表明，在SL的sn-1,3位掺入的C10：0为46.03±0.55mol%和47.28±1.22mol%，分别,用于Lipozyme®RMIM和Lipozyme®435。然而,与Lipozyme®435的50.78±0.44mol%相比,用Lipozyme®RMIM掺入TAG物种中的总C10:0显著更高(60.08±0.04mol%)。放大的(300g)酸解反应和表征在使用Lipozyme®RMIM合成的SL上进行。SL反应产物使用短程蒸馏纯化，并在脂质类别方面充分表征，生育酚,热行为，和氧化稳定性。在短程蒸馏(SPD)之后纯化的放大SL的产率为72.96重量%。SL中的抗氧化剂在SPD后由于生育酚的损失而减少。使用Lipozyme®RMIM在12小时内合成的这种MLM型SL具有高含量的C10：0，并且可能具有功能和健康益处。
