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Abstract：
In this study, hybrid skeleton material ZIF-8@ZIF-67 was synthesized by the epitaxial growth method and then was utilized as a carrier for encapsulating Pseudomonas fluorescens lipase (PFL) through the co-precipitation method, resulting in the preparation of immobilized lipase (PFL@ZIF-8@ZIF-67). Subsequently, it was further treated with glutaraldehyde to improve protein immobilization yield. Under optimal immobilization conditions, the specific hydrolytic activity of PFL@ZIF-8@ZIF-67 was 20.4 times higher than that of the free PFL. The prepared biocatalyst was characterized and analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR). Additionally, the thermal stability of PFL@ZIF-8@ZIF-67 at 50 °C was significantly improved compared to the free PFL. After 7 weeks at room temperature, PFL@ZIF-8@ZIF-67 retained 78% of the transesterification activity, while the free enzyme was only 29%. Finally, PFL@ZIF-8@ZIF-67 was applied to the neryl acetate preparation in a solvent-free system, and the yield of neryl acetate reached 99% after 3 h of reaction. After 10 repetitions, the yields of neryl acetate catalyzed by PFL@ZIF-8@ZIF-67 and the free PFL were 80% and 43%, respectively.
摘要：
在这项研究中,通过外延生长法合成了混合骨架材料ZIF-8@ZIF-67，然后将其用作通过共沉淀法包封荧光假单胞菌脂肪酶（PFL）的载体，从而制备固定化脂肪酶(PFL@ZIF-8@ZIF-67)。随后，进一步用戊二醛处理以提高蛋白质固定化率。在最佳固定条件下，PFL@ZIF-8@ZIF-67的比水解活性是游离PFL的20.4倍。通过扫描电子显微镜(SEM)对所制备的生物催化剂进行了表征和分析,X射线衍射(XRD)傅里叶变换红外(FT-IR)。此外,与游离PFL相比，PFL@ZIF-8@ZIF-67在50°C下的热稳定性显着提高。在室温下放置7周后，PFL@ZIF-8@ZIF-67保留了78％的酯交换活性，而游离酶仅为29%。最后,将PFL@ZIF-8@ZIF-67应用于无溶剂体系中的乙酸金花酯制剂，反应3h后,乙酸金花酯的收率达到99%。重复10次之后,PFL@ZIF-8@ZIF-67和游离PFL催化的乙酸金花酯的收率分别为80%和43%，分别。
