Abstract：
Pathogenic bacteria have consistently posed a formidable challenge to human health, creating the critical need for effective antibacterial solutions. In response, enzyme-metal-organic framework (MOF) composites have emerged as a promising class of antibacterial agents. This study focuses on the development of an enzyme-MOF composite based on HZIF-8, incorporating the advantages of simple synthesis, ZIF-8 antibacterial properties, lysozyme hydrolysis, and high biological safety. Through a one-pot method, core-shell nanoparticles (HZIF-8) were synthesized. This structure enables efficient immobilization of lysozyme and lactoferrin within the HZIF-8, resulting in the formation of the lysozyme-lactoferrin@HZIF-8 (LYZ-LF@HZIF-8) composite. Upon exposure to light irradiation, HZIF-8 itself possessed antibacterial properties. Lysozyme initiated the degradation of bacterial peptidoglycan and lactoferrin synergistically enhanced the antibacterial effect of lysozyme. All of the above ultimately contributed to comprehensive antibacterial activity. Antibacterial assessments demonstrated the efficacy of the LYZ-LF@HZIF-8 composite, effectively eradicating Staphylococcus aureus at a cell density of 1.5 × 106 CFU/mL with a low dosage of 200 μg/mL and completely inactivating Escherichia coli at 400 μg/mL with the same cell density. The enzyme-MOF composite exhibited significant and durable antibacterial efficacy, with no apparent cytotoxicity in vitro, thereby unveiling expansive prospects for applications in the medical and food industries.
摘要：
病原菌一直对人类健康构成巨大挑战，创造对有效抗菌解决方案的关键需求。作为回应,酶-金属-有机骨架（MOF）复合材料已成为一类有前途的抗菌剂。本研究的重点是开发基于HZIF-8的酶-MOF复合材料，结合简单合成的优点，ZIF-8抗菌性能，溶菌酶水解，生物安全性高。通过一锅法，合成了核壳纳米粒子(HZIF-8)。该结构使得溶菌酶和乳铁蛋白能够在HZIF-8内有效固定,导致溶菌酶-乳铁蛋白@HZIF-8(LYZ-LF@HZIF-8)复合物的形成。在暴露于光照射下，HZIF-8本身具有抗菌性能。溶菌酶引发细菌肽聚糖和乳铁蛋白的降解协同增强了溶菌酶的抗菌作用。所有这些最终都有助于全面的抗菌活性。抗菌评估证明了LYZ-LF@HZIF-8复合材料的功效，以200μg/mL的低剂量有效根除1.5×106CFU/mL的金黄色葡萄球菌，以相同的细胞密度完全灭活400μg/mL的大肠杆菌。酶-MOF复合材料表现出显著和持久的抗菌功效，在体外没有明显的细胞毒性，从而揭示了在医疗和食品工业中应用的广阔前景。
