实现高水平的纯化和分离是必不可少的,所以客观粒子,比如恶性细胞,有害细菌,和特殊的蛋白质或生物分子,可以满足药物分析中的高精度测量,临床诊断,靶向治疗,食物防御。此外,这可以揭示个体生物变异的内在本质和进化机制。因此,与光学镊子有关的许多技术,微流体,声电泳,和电动力学可以广泛用于实现微米和纳米尺度的颗粒分离。介电泳(DEP)已用于各种操作,浓度,运输,和生物颗粒的分离过程,由于其早期发展,成熟的理论,低成本,和高吞吐量。尽管许多评论已经讨论了DEP技术的生物学应用,在文献中,对微米级和纳米级颗粒分离特征的全面描述较少。因此,这篇综述总结了粒子分离的现状,关注相关技术的发展和创新,包括理论模拟,微通道结构,电极材料,模式及其布局。此外,还提供了结合使用DEP与其他技术的分离应用的简要概述。最后,结论,未来的指导方针,并强调了潜在晋升的建议。
It is indispensable to realize the high level of purification and separation, so that objective particles, such as malignant cells, harmful bacteria, and special proteins or biological molecules, could satisfy the high precise measurement in the pharmaceutical analysis, clinical diagnosis, targeted therapy, and food defense. In addition, this could reveal the intrinsic nature and evolution mechanisms of individual biological variations. Consequently, many techniques related to optical tweezers, microfluidics, acoustophoresis, and electrokinetics can be broadly used to achieve micro- and nano-scale particle separations. Dielectrophoresis (DEP) has been used for various manipulation, concentration, transport, and separation processes of biological particles owing to its early development, mature theory, low cost, and high throughput. Although numerous reviews have discussed the biological applications of DEP techniques, comprehensive descriptions of micro- and nano-scale particle separations feature less frequently in the literature. Therefore, this review summarizes the current state of particle separation attention to relevant technological developments and innovation, including theoretical simulation, microchannel structure, electrode material, pattern and its layout. Moreover, a brief overview of separation applications using DEP in combination with other technologies is also provided. Finally, conclusions, future guidelines, and suggestions for potential promotion are highlighted.