目的本研究项目的目的是使用彗星测定法评估诊断为宫颈癌的患者的DNA损伤,建立该定量与氧化应激标志物丙二醛(MDA;血浆MDA)之间的相关性,并比较病例和年龄匹配对照之间的结果参数。材料和方法本研究包括49例宫颈癌病例和49例年龄匹配的对照,以测量DNA损伤参数,例如彗星长度,头部直径,彗星头部的DNA百分比,尾部长度,彗星尾部DNA的百分比,和氧化应激标志物(血浆MDA)使用硫代巴比妥酸反应物质(TBARS)酶联免疫吸附测定(ELISA)方法。结果彗星指标表明DNA损伤,如彗星长度,尾部长度,和彗星尾巴中DNA的百分比,宫颈癌病例比年龄匹配的对照组高得多。彗星头部的DNA比例,代表未受损/轻度DNA损伤,年龄匹配的对照组明显高于宫颈癌患者。血浆MDA与彗星尾长呈正相关。与年龄匹配的对照组相比,年龄在30到39岁之间,平价在2到4岁之间,首次怀孕有早年病史和宫颈癌家族史,DNA损伤程度最高.结论彗星参数的升高及其与血浆MDA的正相关,表明诊断为宫颈癌的个体与对照组相比具有更高的DNA损伤程度。结合既定的方法,如PAP涂片,这种包括彗星测定和血浆MDA估计的预测测试可用于识别和评估30-39岁个体的宫颈癌风险,具有2至4次怀孕的胎次,并且在第一次怀孕时具有早期病史,伴有该疾病的阳性家族史。
Purpose The objective of this research project was to estimate DNA damage in patients diagnosed with cervical cancer using the comet assay, establish a correlation between this quantification and the oxidative stress marker malondialdehyde (MDA; plasma MDA), and compare the resulting parameters between the cases and age-matched controls. Materials and methods This study included 49 cervical cancer cases and 49 age-matched controls to measure DNA damage parameters such as comet length, head diameter, percentage of DNA in the comet head, tail length, percentage of DNA in the comet tail, and oxidative stress marker (plasma MDA) using the thiobarbituric acid reactive substance (TBARS) enzyme-linked immunosorbent assay (ELISA) method. Results Comet metrics suggesting DNA damage, such as comet length, tail length, and percentage of DNA in the comet tail, were considerably higher in cervical cancer cases than in age-matched controls. The proportion of DNA in the comet head, representing undamaged/mild DNA damage, was significantly higher in age-matched controls than in cervical cancer patients. Plasma MDA and comet tail length were shown to have a positive correlation. Compared to the age-matched controls, those between the ages of 30 and 39, with a parity of two to four, who had a history of early age at first pregnancy and a positive family history of cervical cancer, had the highest level of DNA damage. Conclusion The elevated levels of comet parameters and their positive correlation with plasma MDA suggest that individuals diagnosed with cervical cancer have a higher degree of DNA damage compared to the control group. In conjunction with established methods like the PAP smear, this predictive test comprising comet assay and estimation of plasma MDA may be utilized to identify and assess the risk of cervical cancer in individuals aged 30-39 years, with a parity between two and four pregnancies and a prior history of early age at first pregnancy, accompanied by a positive family history of the disease.