尽管巴西有各种各样的本地和外来水果,对它们在储存过程中支持病原体的能力了解有限。这项研究旨在评估接种到巴西本土和异国情调的八种水果果肉中的肠道沙门氏菌和单核细胞增生李斯特菌的行为:Jenipa(GenipaamericanaL.),Umbu(SpondiastuberosaArruda),Maná(Solanumsessiliflorum),Cajá-manga(Spondiasdulcis),酸浆(PhysalisangulataL.),Feijoa(Accasellowiana),Cupuaçu(Theobromagrandiflorum)(平均pH<3.3)和低酸果实:Abiu(Pouteriacaimito)(pH6.11)。将病原体接种到不同的水果中,并在10、20、30和37°C下储存长达12小时和6天,分别。在评估的水果中,阿比乌是唯一允许沙门氏菌生长的,在20和30°C下显示较高的δ值(对于两个温度均为5.6logCFU/g)。对于Physalis和Feijoa,病原体浓度略有降低(<1个对数周期),主要在10和20°C,表明其保持在矩阵中的能力。对于其他水果,获得了显著的负δ值,表明微生物失活的趋势。阿比乌的生存潜力受到温度的显着影响,Maná,库普阿苏,和Cajá-manga(p<0.05)。对于单核细胞增生李斯特菌群体观察到关于δ值的相同现象,在Abiu中在20°C下观察到最大的生存潜力(3.3logCFU/g)。关于阿比乌的指数增长率,在30和37°C时观察到最高值,沙门氏菌(4.6和4.9log(CFU/g)/天,分别)和单核细胞增生李斯特菌(2.8和2.7log(CFU/g)/天,分别),两个温度之间没有显着差异。关于微生物灭活,实际上,在所有基质中,单核细胞增生李斯特菌都比沙门氏菌表现出更高的抗性。Jenipapo和Umbu是纸浆,总的来说,对减少病原体种群的影响最大。此外,储存温度的增加似乎有利于失活速率的增加。总之,沙门氏菌和单核细胞增生李斯特菌只能在阿比乌纸浆中生长,尽管它们可以在冷藏和恶劣温度下保存的一些酸性热带水果中存活。
Despite the wide variety of native and exotic fruits in Brazil, there is limited understanding of their ability to support pathogens during storage. This study aimed to evaluate the behavior of Salmonella enterica and Listeria monocytogenes inoculated into the pulp of eight fruits native and exotic to Brazil: Jenipapo (Genipa americana L.), Umbu (Spondias tuberosa Arruda), Maná (Solanum sessiliflorum), Cajá-manga (Spondias dulcis), Physalis (Physalis angulata L.), Feijoa (Acca sellowiana), Cupuaçu (Theobroma grandiflorum) (average pH < 3.3) and in a low acidy fruit: Abiu (Pouteria caimito) (pH 6.11). The pathogens were inoculated into the different fruits and stored at 10, 20, 30 and 37 °C for up to 12 h and 6 days, respectively. Among the fruits evaluated, Abiu was the only one that allowed Salmonella growth, showing higher δ-values at 20 and 30 °C (5.6 log CFU/g for both temperatures). For Physalis and Feijoa, there was a small reduction in the pathogen concentration (<1 log-cycle), mainly at 10 and 20 °C, indicating its ability to remain in the matrices. For the other fruits, notable negative δ-values were obtained, indicating a tendency towards microbial inactivation. The survival potential was significantly affected by temperature in Abiu, Maná, Cupuaçu, and Cajá-manga (p < 0.05). The same phenomena regarding δ-value were observed for L. monocytogenes population, with the greatest survival potential observed at 20 °C in Abiu (3.3 log CFU/g). Regarding the exponential growth rates in Abiu, the highest values were observed at 30 and 37 °C, both for Salmonella (4.6 and 4.9 log (CFU/g)/day, respectively) and for L. monocytogenes (2.8 and 2.7 log (CFU/g)/day, respectively), with no significant difference between both temperatures. Regarding microbial inactivation, L. monocytogenes showed greater resistance than Salmonella in practically all matrices. Jenipapo and Umbu were the pulps that, in general, had the greatest effect on reducing the population of pathogens. Furthermore, the increase in storage temperature seems to favor the increase on inactivation rates. In conclusion, Salmonella and L. monocytogenes can grow only in Abiu pulp, although they can survive in some acidic tropical fruits kept at refrigeration and abusive temperatures.