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Abstract：
OBJECTIVE: To evaluate the protective effect of an experimental solution containing TiF4/NaF on the development of radiation-induced dentin caries lesions.
METHODS: bovine root samples were irradiated (70Gy) and distributed as following (n=12/group): Commercial Saliva (BioXtra), NaF (500 ppm F-), TiF4 (500 ppm F), TiF4/NaF (TiF4: 300 ppm F-, NaF: 190 ppm F-), and Phosphate buffer solution (PBS, negative control). Biofilm was produced using biofilm from irradiated patients and McBain saliva (0.2% of sucrose, at 37oC and 5% CO2) for five days. The treatments were applied 1x/day. Colony-forming units (CFU) were counted and demineralization was quantified by transversal microradiography. The ANOVA/Tukey test was applied for all parameters.
RESULTS: All treatments reduced CFU for total microorganisms. TiF4 reduced Lactobacillus sp. (7.04±0.26 log10 CFU/mL) and mutans streptococci (7.18±0.28) CFU the most, when compared to PBS (7.58±0.21 and 7.75±0.17) and followed by NaF (7.12±0.31 and 7.34±0.22) and TiF4/NaF (7.16±0.35 and 7.29± 0.29). TiF4 and Commercial saliva showed the lowest integrated mineral loss (ΔZ-vol%.mm) (1977±150 and 2062±243, respectively) when compared to PBS (4540±335), followed by NaF (2403±235) and TiF4/NaF (2340±200). Commercial saliva was the only to significantly reduce mineral loss (LD-µm) (111±25) compared to PBS (153±24).Mean mineral loss (R-vol%) decreased by 35.2% for TiF4 (18.2±3.3) when compared to PBS (28.1±2.9) Conclusion: TiF4/NaF has a comparable anti-cariogenic effect to TiF4 and Commercial saliva under the model in this study.
METHODS: bovine root samples were irradiated (70Gy) and distributed as following (n=12/group): Commercial Saliva (BioXtra), NaF (500 ppm F-), TiF4 (500 ppm F), TiF4/NaF (TiF4: 300 ppm F-, NaF: 190 ppm F-), and Phosphate buffer solution (PBS, negative control). Biofilm was produced using biofilm from irradiated patients and McBain saliva (0.2% of sucrose, at 37oC and 5% CO2) for five days. The treatments were applied 1x/day. Colony-forming units (CFU) were counted and demineralization was quantified by transversal microradiography. The ANOVA/Tukey test was applied for all parameters.
RESULTS: All treatments reduced CFU for total microorganisms. TiF4 reduced Lactobacillus sp. (7.04±0.26 log10 CFU/mL) and mutans streptococci (7.18±0.28) CFU the most, when compared to PBS (7.58±0.21 and 7.75±0.17) and followed by NaF (7.12±0.31 and 7.34±0.22) and TiF4/NaF (7.16±0.35 and 7.29± 0.29). TiF4 and Commercial saliva showed the lowest integrated mineral loss (ΔZ-vol%.mm) (1977±150 and 2062±243, respectively) when compared to PBS (4540±335), followed by NaF (2403±235) and TiF4/NaF (2340±200). Commercial saliva was the only to significantly reduce mineral loss (LD-µm) (111±25) compared to PBS (153±24).Mean mineral loss (R-vol%) decreased by 35.2% for TiF4 (18.2±3.3) when compared to PBS (28.1±2.9) Conclusion: TiF4/NaF has a comparable anti-cariogenic effect to TiF4 and Commercial saliva under the model in this study.
摘要：
目的：评估含TiF4/NaF的实验溶液对辐射诱导的牙本质龋齿病变发展的保护作用。
方法：对牛根样品进行辐照（70Gy）并按以下方式分布（n=12/组）：商业唾液（BioXtra），NaF(500ppmF-),TiF4(500ppmF),TiF4/NaF(TiF4:300ppmF-,NaF:190ppmF-),和磷酸盐缓冲溶液(PBS，阴性对照)。生物膜是使用受照射患者的生物膜和McBain唾液（0.2％的蔗糖，在37oC和5%CO2下)持续五天。处理施用1x/天。计数菌落形成单位（CFU），并通过横向显微照相定量脱矿质。对所有参数应用ANOVA/Tukey检验。
结果：所有处理均降低了总微生物的CFU。TiF4减少乳杆菌属。(7.04±0.26log10CFU/mL)和变形链球菌(7.18±0.28)CFU最,与PBS（7.58±0.21和7.75±0.17）相比，然后是NaF（7.12±0.31和7.34±0.22）和TiF4/NaF（7.16±0.35和7.29±0.29）。TiF4和商业唾液显示出最低的整合矿物质损失(ΔZ-vol%。mm）（分别为1977±150和2062±243），与PBS（4540±335）相比，其次是NaF(2403±235)和TiF4/NaF(2340±200)。与PBS（153±24）相比，商业唾液是唯一显着降低矿物质损失（LD-μm）（111±25）的方法。当与PBS(28.1±2.9)相比时,TiF4(18.2±3.3)的平均矿物质损失(R体积%)降低了35.2%。结论:在本研究的模型下,TiF4/NaF具有与TiF4和商业唾液相当的抗致龋作用。
方法：对牛根样品进行辐照（70Gy）并按以下方式分布（n=12/组）：商业唾液（BioXtra），NaF(500ppmF-),TiF4(500ppmF),TiF4/NaF(TiF4:300ppmF-,NaF:190ppmF-),和磷酸盐缓冲溶液(PBS，阴性对照)。生物膜是使用受照射患者的生物膜和McBain唾液（0.2％的蔗糖，在37oC和5%CO2下)持续五天。处理施用1x/天。计数菌落形成单位（CFU），并通过横向显微照相定量脱矿质。对所有参数应用ANOVA/Tukey检验。
结果：所有处理均降低了总微生物的CFU。TiF4减少乳杆菌属。(7.04±0.26log10CFU/mL)和变形链球菌(7.18±0.28)CFU最,与PBS（7.58±0.21和7.75±0.17）相比，然后是NaF（7.12±0.31和7.34±0.22）和TiF4/NaF（7.16±0.35和7.29±0.29）。TiF4和商业唾液显示出最低的整合矿物质损失(ΔZ-vol%。mm）（分别为1977±150和2062±243），与PBS（4540±335）相比，其次是NaF(2403±235)和TiF4/NaF(2340±200)。与PBS（153±24）相比，商业唾液是唯一显着降低矿物质损失（LD-μm）（111±25）的方法。当与PBS(28.1±2.9)相比时,TiF4(18.2±3.3)的平均矿物质损失(R体积%)降低了35.2%。结论:在本研究的模型下,TiF4/NaF具有与TiF4和商业唾液相当的抗致龋作用。
