尽管蝙蝠(哺乳动物:Chiroptera)是世界上许多人畜共患病原体的天然储库,很少有研究调查蝙蝠中无性子科病原体的发生,尤其是吸血蝙蝠.无性体科(立克次体科)包括无性体属的专性细胞内细菌,埃里希亚,Neorickettsia,Neoehrlichia,Wolbachia,和Allocryplasma。本研究旨在调查,使用分子技术,无性体物种的存在,埃里希亚,以及巴西北部吸血蝙蝠的Neorickettsia。在2017年至2019年之间,从属于两个物种的吸血蝙蝠中收集了脾脏样本,来自帕拉州(n=207)的Desmodusrotundus(n=228),亚马逊(n=1),罗赖马(n=18)和阿马帕(n=3),和来自帕拉的Diaemusyoungii(n=1)。阳性率5.2%(12/229),3%(7/229),在Anaplasma属的PCR检测中发现10.9%(25/229)。(16SrRNA基因),埃里希菌属。(dsb基因)和Neorickettsiaspp。(16SrRNA基因),分别。本研究显示,第一次,无性体的发生。和不同基因型的埃里希菌属。来自巴西的吸血蝙蝠。而基于埃里希菌的dsb和ftsZ基因和无性体的16SrRNA的系统发育分析。揭示了在吸血蝙蝠中检测到的基因型与与国内反刍动物相关的无性体科病原体的系统发育接近性,基于gltA和groEL基因的系统发育推断证明了蝙蝠明显专有的基因型的发生。Neorickettsiasp.在巴西北部的吸血蝙蝠中也发现了与N.risticii相关的系统发育。
Although bats (Mammalia:
Chiroptera) act as natural reservoirs for many zoonotic pathogens around the world, few studies have investigated the occurrence of Anaplasmataceae agents in bats, especially vampire bats. The family Anaplasmataceae (order Rickettsiales) encompasses obligate intracellular bacteria of the genera Anaplasma, Ehrlichia, Neorickettsia, Neoehrlichia, Wolbachia, and Allocryptoplasma. The present study aimed to investigate, using molecular techniques, the presence of species of Anaplasma, Ehrlichia, and Neorickettsia in vampire bats sampled in northern Brazil. Between 2017 and 2019, spleen samples were collected from vampire bats belonging to two species, Desmodus rotundus (n = 228) from the states of Pará (n = 207), Amazonas (n = 1), Roraima (n = 18) and Amapá (n = 3), and Diaemus youngii (n = 1) from Pará. Positivity rates of 5.2% (12/229), 3% (7/229), and 10.9% (25/229) were found in PCR assays for Anaplasma spp. (16S rRNA gene), Ehrlichia spp. (dsb gene) and Neorickettsia spp. (16S rRNA gene), respectively. The present study revealed, for the first time, the occurrence of Anaplasma spp. and different genotypes of Ehrlichia spp. in vampire bats from Brazil. While phylogenetic analyses based on the dsb and ftsZ genes of Ehrlichia and 16S rRNA of Anaplasma spp. revealed phylogenetic proximity of the genotypes detected in vampire bats with Anaplasmataceae agents associated with domestic ruminants, phylogenetic inferences based on the gltA and groEL genes evidenced the occurrence of genotypes apparently exclusive to bats. Neorickettsia sp. phylogenetically associated with N. risticii was also detected in vampire bats sampled in northern Brazil.