背景:Chayote是葫芦科的高经济作物,在粮食生产中发挥着重要作用,疾病治疗和工业可降解材料的生产。由于环境恶劣,如高温,干旱和霜冻,一些佛手瓜资源正在逐渐消失。重要的是收集,表征,并保存佛手球资源。然而,到目前为止,尚未对中国佛手瓜资源的遗传多样性进行研究。
结果:在这项研究中,我们从中国14个省收集了35只佛手瓜。随后,我们从佛手瓜基因组中发现了363,156个SSR基序,并设计了57对SSR引物进行验证。在这些中,48对引物成功扩增条带,其中42个显示多态性。这42对引物共检测到153个等位基因,每个基因座平均3.64个等位基因。多态信息含量范围为0.03至0.78,平均值为0.41,表明多态性水平较高。基于使用结构分析,PCoA,和UPGMA方法,这35个佛手瓜个体被分为两个主要的集群。通过进一步的关联分析,确定了7个显著相关的SSR标记,包括四个与皮颜色有关,三个与脊柱有关。
结论:这些分子标记将有助于将来佛手瓜的遗传多样性分析和遗传育种改进。
BACKGROUND: Chayote is a high economic crop in the Cucurbitaceae family, playing an important role in food production, disease treatment and the production of degradable materials in industries. Due to the harsh environment, such as high temperature, drought and frost, some chayote resources are gradually disappearing. It is crucial to collect, characterize, and conserve chayote resources. However, the genetic diversity of chayote resources in China has not been studied so far.
RESULTS: In this study, we collected 35 individuals of chayote from 14 provinces in China. Subsequently, we found 363,156 SSR motifs from the chayote genome and designed 57 pairs of SSR primers for validation. Out of these, 48 primer pairs successfully amplified bands, with 42 of them showing polymorphism. These 42 primer pairs detected a total of 153 alleles, averaging 3.64 alleles per locus. The polymorphic information content ranged from 0.03 to 0.78, with an average value of 0.41, indicating a high level of polymorphism. Based on the analysis using STRUCTURE, PCoA, and UPGMA methods, the 35 chayote individuals were divided into two major clusters. Through further association analysis, 7 significantly associated SSR markers were identified, including four related to peel color and three related to spine.
CONCLUSIONS: These molecular markers will contribute to the analysis of genetic diversity and genetic breeding improvement of chayote in the future.