杂合性(ROHet)和纯合性(ROH)的运行包含与目的性状相关的有用信息。缺乏研究ROHet和ROH对哺乳动物繁殖成功和繁殖损失的影响。这里,我们检测并表征了中国本地猪基因组中的ROHet和ROH模式(即,金华,春安,LongyouBlack,和圣贤斑猪),揭示了本地猪的相似遗传特征。稍后,在这四个本地猪品种中,我们使用线性模型的关联分析强调了与落叶性状相关的ROHet和ROH。为了确定与凋落物性状相关的有希望的候选基因,我们进一步深入探讨了其他五个猪品种的选择模式(即,Erhualian,眉山,Minzhu,荣昌,和迪庆猪),使用FST和遗传多样性比,在与基本凋落物性状相关的ROHet和ROH上具有不同水平的繁殖性能。然后,我们鉴定了一组与猪繁殖性能相关的已知和新的候选基因。对于新的候选基因(即,CCDC91,SASH1,SAMD5,MACF1,MFSD2A,EPC2和MBD5),我们获得了公开可用的数据集,并进行了多组学分析,整合了转录组范围的关联研究和比较性单细胞RNA-seq分析,以揭示它们在哺乳动物生殖表现中的作用.这些基因尚未被广泛报道为与生育力相关的基因,并且可以补充地视为先前的生物学信息,以修饰有利于猪产仔性状遗传改善的基因组选择模型。此外,我们的发现为ROHet和ROH在哺乳动物中的功能提供了新的见解。
Runs of heterozygosity (ROHet) and homozygosity (ROH) harbor useful information related to traits of interest. There is a lack of investigating the effect of ROHet and ROH on reproductive success and the loss of reproduction in mammals. Here, we detected and characterized the ROHet and ROH patterns in the genomes of Chinese indigenous pigs (i.e., Jinhua, Chun\'an, Longyou Black, and Shengxian Spotted pigs), revealing the similar genetic characteristics of indigenous pigs. Later, we highlighted the underlying litter traits-related ROHet and ROH using association analysis with linear model in these four indigenous pig breeds. To pinpoint the promising candidate genes associated with litter traits, we further in-depth explore the selection patterns of other five pig breeds (i.e., Erhualian, Meishan, Minzhu, Rongchang, and Diqing pigs) with different levels of reproduction performance at the underlying litter traits-related ROHet and ROH using F ST and genetic diversity ratio. Then, we identified a set of known and novel candidate genes associated with reproductive performance in pigs. For the novel candidate genes (i.e., CCDC91, SASH1, SAMD5, MACF1, MFSD2A, EPC2, and MBD5), we obtained public available datasets and performed multi-omics analyses integrating transcriptome-wide association studies and comparative single-cell RNA-seq analyses to uncover the roles of them in mammalian reproductive performance. The genes have not been widely reported to be fertility-related genes and can be complementally considered as prior biological information to modify genomic selections models that benefits pig genetic improvement of litter traits. Besides, our findings provide new insights into the function of ROHet and ROH in mammals.