PDF(Pubmed)
Abstract：
The association between pre-harvest sprouting (PHS) and seed coat color has long been recognized. Red-grained wheats generally exhibit greater PHS resistance compared to white-grained wheat, although variability in PHS resistance exists within red-grained varieties. Here, we conducted a genome-wide association study on a panel consisting of red-grained wheat varieties, aimed at uncovering genes that modulate PHS resistance and red color components of seed coat using digital image processing. Twelve loci associated with PHS traits were identified, nine of which were described for the first time. Genetic loci marked by SNPs AX-95172164 (chromosome 1B) and AX-158544327 (chromosome 7D) explained approximately 25% of germination index variance, highlighting their value for breeding PHS-resistant varieties. The most promising candidate gene for PHS resistance was TraesCS6B02G147900, encoding a protein involved in aleurone layer morphogenesis. Twenty-six SNPs were significantly associated with grain color, independently of the known Tamyb10 gene. Most of them were related to multiple color characteristics. Prioritization of genes within the revealed loci identified TraesCS1D03G0758600 and TraesCS7B03G1296800, involved in the regulation of pigment biosynthesis and in controlling pigment accumulation. In conclusion, our study identifies new loci associated with grain color and germination index, providing insights into the genetic mechanisms underlying these traits.
摘要：
长期以来，人们已经认识到收获前发芽（PHS）与种皮颜色之间的关联。与白粒小麦相比，红粒小麦通常表现出较高的PHS抗性，尽管红粒品种中存在PHS抗性的变异性。这里,我们对由红粒小麦品种组成的小组进行了全基因组关联研究，旨在使用数字图像处理技术发现调节PHS抗性和种皮红色成分的基因。确定了与PHS性状相关的12个基因座，其中9个是第一次描述。由SNPAX-95172164（染色体1B）和AX-158544327（染色体7D）标记的遗传基因座解释了大约25％的发芽指数变异，突出了它们对培育抗PHS品种的价值。PHS抗性最有希望的候选基因是TraesCS6B02G147900，编码参与糊粉层形态发生的蛋白质。26个SNP与谷物颜色显着相关，独立于已知的Tamyb10基因。它们中的大多数与多种颜色特征有关。揭示的基因座内的基因优先级确定了TraesCS1D03G0758600和TraesCS7B03G1296800，它们参与调节色素生物合成和控制色素积累。总之,我们的研究确定了与谷物颜色和发芽指数相关的新基因座，提供对这些性状的遗传机制的见解。
