常见细菌大肠杆菌在生物降解领域显示出潜力。大肠杆菌天然能够生物降解,因为它携带各种酶,这些酶对于分解不同的物质至关重要。降解过程由这些酶有效催化。E.的协作效应大肠杆菌芳基磺基转移酶,链烷磺酸月亮加氧酶,在这项工作中,研究了偶氮还原酶对工业废水中硫化染料分解的作用。ExPASYProtParam用于确认酶的稳定性,显示不稳定指数小于40。我们确定了这些酶与硫化染料污染物-1-萘磺酸的最大结合亲和力,磺基基因,硫磺绿3,硫磺红6,硫磺红1,硫磺黄2,噻吩,硫嗪酮,和硫基-使用比较分子对接。重要的是,单加氧酶(-12.1)显示出最高的结合亲和力,而芳基磺基转移酶和偶氮还原酶分别表现出-11.8和-11.4的显着能量。检查了对接复合物中蛋白质与配体之间的相互作用。为了评估它们的综合效果,进行了基因共表达分析和酶生物工程。使用芳基磺基转移酶,链烷磺酸盐单加氧酶,和偶氮还原酶,这项研究调查了硫磺染料污染物的酶降解,从而促进环境友好和有效的硫化染料污染物管理。
The common bacterium Escherichia coli has demonstrated potential in the field of biodegradation. E. coli is naturally capable of biodegradation because it carries a variety of enzymes that are essential for the breakdown of different substances. The degradation process is effectively catalyzed by these enzymes. The collaborative effects of E. coli\'s aryl sulfotransferase, alkanesulfonate moonoxygenase, and azoreductase enzymes on the breakdown of sulfur dyes from industrial effluents are investigated in this work. ExPASY ProtParam was used to confirm the stability of the enzyme, showing an instability index less than 40. We determined the maximum binding affinities of these enzymes with sulfur dye pollutants - 1-naphthalenesulfonic acid, sulfogene, sulfur green 3, sulfur red 6, sulfur red 1, sulfur yellow 2, thianthrene, thiazone, and thional - using comparative molecular docking. Significantly, the highest binding affinity was shown by monooxygenase (-12.1), whereas aryl sulfotransferase and azoreductase demonstrated significant energies of -11.8 and -11.4, respectively. The interactions between proteins and ligands in the docked complexes were examined. To evaluate their combined effects, co-expression analysis of genes and enzyme bioengineering were carried out. Using aryl sulfotransferase, alkanesulfonate monooxygenase, and azoreductase, this study investigates the enzymatic degradation of sulfur dye pollutants, thereby promoting environmentally friendly and effective sulfur dye pollutant management.