背景:世界上超过50%的人口感染了幽门螺杆菌(H。pylori),被世界卫生组织列为第一类致癌物。
结果:对玉米蛋白双功能肽进行了鉴定,并在体外和计算机上进行了功能分析。两种新的双功能肽被鉴定为Cys-Gln-Asp-Val-Pro-Leu-Leu(CQDVPLL,CQ7)和Thr-Ile-Phe-Pro-Gln-Cys(TIFPQC,TI6)纳米LC-MS/MS在4mg/mL时,CQ7和TI6对幽门螺杆菌的抗粘附活性分别为45.17±2.41%和48.62±1.84%,分别。计算机预测表明CQ7和TI6具有良好的理化性质。分子对接表明,CQ7和TI6可以通过疏水相互作用和氢键与BabA和SabA结合,预防幽门螺杆菌感染。此外,CQ7由于其独特的氨基酸组成而显示出强的抗氧化活性。
结论:本研究表明,已鉴定的肽CQ7和TI6具有抗氧化和抗粘附作用,预防幽门螺杆菌感染以及减轻对胃粘膜的氧化损伤。本文受版权保护。保留所有权利。
BACKGROUND: More than 50% of the world\'s population is infected with Helicobacter pylori, which is classified as a group I carcinogen by the World Health Organization (WHO).
RESULTS: Corn protein dual-functional peptides were identified and functionally analyzed in vitro and in silico. Two novel dual-functional peptides were identified as Cys-Gln-Asp-Val-Pro-Leu-Leu (CQDVPLL, CQ7) and Thr-Ile-Phe-Pro-Gln-Cys (TIFPQC, TI6) using nanoscale liquid chromatography coupled to tandem mass spectrometry (nano-LC-MS/MS). The antiadhesive effects against H. pylori of CQ7 and TI6 were 45.17 ± 2.41% and 48.62 ± 1.84% at 4 mg mL-1 , respectively. In silico prediction showed that CQ7 and TI6 had good physicochemical properties. Molecular docking demonstrated that CQ7 and TI6 could bind to the adhesins BabA and SabA by hydrophobic interactions and hydrogen bonds, preventing H. pylori infection. Moreover, CQ7 showed strong antioxidant activity due to its unique amino acid composition.
CONCLUSIONS: The present study demonstrated that the identified peptides, CQ7 and TI6, possess antioxidant and antiadhesive effects, preventing H. pylori infection and alleviating oxidative injury to the gastric mucosa. © 2023 Society of Chemical Industry.