双壳类动物可以浓缩生物和化学污染物,导致食源性爆发,其发生率正在增加,由于气候和人类因素难以逆转,因此需要改进监控。这项研究旨在评估西西里岛生产和分销链中采样的双壳类动物的卫生质量,并收集有用的数据以确保消费者安全。对254个双壳类样品进行了细菌学和分子分析,以检测肠致病性弧菌,弓形虫。,气单胞菌属。,沙门氏菌属。,和β-葡糖醛酸酶阳性大肠杆菌。254个样本中总共有96个,在生产区域收集,用于藻类生物毒素和重金属检测。还评估了来自水产养殖植入物的21个水样品的细菌和藻类污染。弧菌属。,弓形虫。,嗜水气单胞菌,沙门氏菌属。,在106/254、79/254、12/254、16/254和95/254软体动物中检测到大肠杆菌,分别。共有10/96双壳类动物的藻类生物毒素检测呈阳性,金属在法定限额之内。五、溶藻,A.butzleri,在5、3和3个水样中检测到大肠杆菌,分别。Alexandriumminutum,尖锐湿疣,柳叶刀,和假单胞菌。在用含生物毒素的软体动物收集的水样中检测到。从含有相应的生产藻类的水样中的软体动物中检测到了痕量的叶素毒素。尽管欧盟委员会对贝类供应链监控进行了严格的监管,我们的分析强调了提高效率的必要性。
Bivalves can concentrate biological and chemical pollutants, causing foodborne outbreaks whose occurrence is increasing, due to climatic and anthropic factors that are difficult to reverse, hence the need for improved surveillance. This study aimed to evaluate the hygienic qualities of bivalves sampled along the production and distribution chain in Sicily and collect useful data for consumer safety. Bacteriological and molecular analyses were performed on 254 samples of bivalves for the detection of enteropathogenic Vibrio, Arcobacter spp., Aeromonas spp., Salmonella spp., and beta-glucuronidase-positive Escherichia coli. A total of 96 out of 254 samples, collected in the production areas, were processed for algal biotoxins and heavy metals detection. Bacterial and algal contaminations were also assessed for 21 samples of water from aquaculture implants. Vibrio spp., Arcobacter spp., Aeromonas hydrophila, Salmonella spp., and Escherichia coli were detected in 106/254, 79/254, 12/254, 16/254, and 95/254 molluscs, respectively. A total of 10/96 bivalves tested positive for algal biotoxins, and metals were under the legal limit. V. alginolyticus, A. butzleri, and E. coli were detected in 5, 3, and 3 water samples, respectively. Alexandrium minutum, Dinophysis acuminata, Lingulodinium polyedra, and Pseudonitzschia spp. were detected in water samples collected with the biotoxin-containing molluscs. Traces of yessotoxins were detected in molluscs from water samples containing the corresponding producing algae. Despite the strict regulation by the European Commission over shellfish supply chain monitoring, our analyses highlighted the need for efficiency improvement.