本研究旨在评估抗菌素耐药性,毒力,广西候鸟气单胞菌的遗传多样性,广东省,宁夏回族自治区,江西省,和中国的内蒙古。总共收集了810个样本,包括新鲜的粪便,泄殖腔拭子,和喉咙拭子。处理收集的样品并进行细菌学检查。评估了对21种抗生素的耐药性。使用串联的gltA-groL-gyrB-metG-PPSA-recA序列构建了系统发育树。通过PCR和测序鉴定了8个推定的毒力因子,并使用改良的微量滴定板法进行生物膜形成测定。总的来说,分离出176株气单胞菌,包括沙菌,A.亲水物,A.veronii,还有A.caviae.所有分离株对所有16种测试的抗生素圆盘显示出可变的抗性,只有一种抗生素没有参考标准。发现了六种毒力基因标记,检出率为46.0%(hlyA),76.1%(aerA),52.3%(alt),4.5%(最后),54.0%(FLA),和64.2%(唇)。这些菌株能够形成不同大小的生物膜;102个弱粘附,14人中等粘附,60人没有粘附,没有人强烈坚持。我们的结果表明,候鸟携带高毒力和多重耐药性的气单胞菌,并通过迁徙传播到世界各地,这是对公众健康的潜在威胁。
This study aimed to evaluate antimicrobial resistance, virulence, and the genetic diversity of Aeromonas isolated from migratory birds from Guangxi Province, Guangdong Province, Ningxia Hui Autonomous Region, Jiangxi Province, and Inner Mongolia in
China. A total of 810 samples were collected, including fresh feces, cloacal swabs, and throat swabs. The collected samples were processed and subjected to bacteriological examination. The resistance to 21 antibiotics was evaluated. A phylogenetic tree was constructed using concatenated gltA-groL-gyrB-metG-PPSA-recA sequences. Eight putative virulence factors were identified by PCR and sequencing, and a biofilm formation assay was performed using a modified microtiter plate method. In total, 176 Aeromonas isolates were isolated including A. sobria, A. hydrophila, A. veronii, and A. caviae. All isolates showed variable resistance against all 16 tested antibiotic discs, and only one antibiotic had no reference standard. Six kinds of virulence gene markers were discovered, and the detection rates were 46.0% (hlyA), 76.1% (aerA), 52.3% (alt), 4.5% (ast), 54.0% (fla), and 64.2% (lip). These strains were able to form biofilms with distinct magnitudes; 102 were weakly adherent, 14 were moderately adherent, 60 were non-adherent, and none were strongly adherent. Our results suggest that migratory birds carry highly virulent and multidrug-resistant Aeromonas and spread them around the world through migration, which is a potential threat to public health.