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Abstract：
This study aimed to characterize 300 Aeromonas spp. strains isolated from 123 ornamental fish of 32 different species presenting with septicemia, skin lesions, and/or eye lesions. Within the 300 strains, 53.0% were identified as A. veronii, 41.3% as A. hydrophila, and 5.7% as A. caviae. Among the six virulence genes investigated, the most frequent were act (90.3%) and aer (79.3%). More than 50% of A. hydrophila strains were positive for all the studied genes. A total of 30 virulence profiles were identified, with the five main profiles identified comprising 75% of strains. Only five strains were negative for all genes and were identified as A. caviae and A. veronii. The antimicrobial susceptibility profile was performed for 234 strains, with sulfonamides presenting more than 50% of the resistance rates. Susceptibility was observed mainly for cephalosporins, aminoglycosides, chloramphenicol and piperacillin-tazobactam. Multidrug resistance was detected in 82.5% of the studied strains, including A. caviae with 100% multidrug resistance, and A. hydrophila with 90.9% multidrug resistance. The SE-AFLP analysis resulted in 66 genotypes of A. hydrophila, 118 genotypes of A. veronii, and 14 genotypes of A. caviae, demonstrating the greater heterogeneity of A. veronii and A. caviae. However, no direct correlation was observed between the genotypes and the strains\' origins or virulence and resistance profiles.
摘要：
本研究旨在表征300个气单胞菌属。从32种不同物种的123种观赏鱼中分离出的菌株，皮肤损伤,和/或眼部病变。在300个菌株中，53.0%的人被鉴定为A.veronii，41.3%为嗜水气单胞菌，和5.7%为鱼腥草。在研究的六个毒力基因中，最常见的是行为（90.3％）和aer（79.3％）。超过50%的嗜水气单胞菌菌株对所有研究的基因均为阳性。共鉴定出30个毒力谱,确定的五个主要概况包括75%的菌株。只有五个菌株对所有基因均为阴性，并被鉴定为A.caviae和A.veronii。对234个菌株进行了抗菌药物敏感性分析，磺胺类药物的耐药率超过50%。主要观察到头孢菌素的易感性，氨基糖苷类,氯霉素和哌拉西林他唑巴坦.在82.5%的研究菌株中检测到多药耐药性,包括具有100%多药耐药性的A.cavia,和具有90.9%多药耐药性的嗜水菌。SE-AFLP分析产生了66个基因型的嗜水气单胞菌，118基因型的A.veronii，和14个基因型的鱼腥草，证明了维龙氏A和鱼腥草的异质性更大。然而,基因型与菌株起源或毒力和抗性谱之间未观察到直接相关。
