{Reference Type}: Journal Article
{Title}: Municipal wastewater monitoring revealed the predominance of bla GES genes with diverse variants among carbapenemase-producing organisms: high occurrence and persistence of Aeromonas caviae harboring the new bla GES variant bla GES-48.
{Author}: Tanabe M;Sugawara Y;Denda T;Sakaguchi K;Takizawa S;Koide S;Hayashi W;Yu L;Kayama S;Sugai M;Nagano Y;Nagano N;
{Journal}: Microbiol Spectr
{Volume}: 11
{Issue}: 6
{Year}: 2023 Dec 12
{Factor}: 9.043
{DOI}: 10.1128/spectrum.02188-23
{Abstract}: <B>OBJECTIVE: </B>The emergence and spread of carbapenemase-producing organisms (CPOs) represent a global health threat because they are associated with limited treatment options and poor clinical outcomes. Wastewater is considered a hotspot for the evolution and dissemination of antimicrobial resistance. Thus, analyses of municipal wastewater are critical for understanding the circulation of these CPOs and carbapenemase genes in local communities, which remains scarcely known in Japan. This study resulted in several key observations: (i) the vast majority of bla GES genes, including six new bla GES variants, and less frequent bla IMP genes were carbapenemase genes encountered exclusively in wastewater influent; (ii) the most dominant CPO species were Aeromonas spp., in which a remarkable diversity of new sequence types was observed; and (iii) CPOs were detected from combined sewer wastewater, but not from separate sewer wastewater, suggesting that the load of CPOs from unrecognized environmental sources could greatly contribute to their detection in influent wastewater.