褐腐病,由Moniliniaspp引起的。,对全球梨果和核果作物构成重大威胁,在收获前和收获后阶段造成巨大的经济损失。果莫尼氏菌,M.laxa,和果果分枝杆菌被确定为负责褐腐病的关键因子。在这项研究中,我们采用扩增片段长度多态性(AFLP)方法评估了86株Moniliniaspp的遗传多样性。从韩国主要的核果种植地区分离。具体来说,菌株是从忠清收集的,江原,京流,庆尚,全罗省(-do)。应变特性的比较分析,例如隔离位置,寄主植物,以及对化学杀菌剂的反应,进行了。使用20对引物的AFLP系统发育分类显示存在三个不同的群体,全罗省的菌株一直以高频率形成一个单独的组。此外,通过AFLP模式将果蝇分为三组。主坐标分析和PERMANOVA用于比较应变信息,如起源,host,和杀菌剂的敏感性,根据地理起源和寄主植物揭示了AFLP的重要分区模式。这项研究代表了AFLP方法的利用,以调查果聚糖分离株之间的遗传变异,强调持续监测和管理褐腐病原体变异的重要性。
Brown rot disease, caused by Monilinia spp., poses a significant threat to pome and stone fruit crops globally, resulting in substantial economic losses during pre- and post-harvest stages. Monilinia fructigena, M. laxa, and M. fructicola are identified as the key agents responsible for brown rot disease. In this study, we employed the amplified fragment length polymorphism (
AFLP) method to assess the genetic diversity of 86 strains of Monilinia spp. isolated from major stone fruit cultivation regions in South Korea. Specifically, strains were collected from Chungcheong, Gangwon, Gyeonggi, Gyeongsang, and Jeolla provinces (-do). A comparative analysis of strain characteristics, such as isolation locations, host plants, and responses to chemical fungicides, was conducted.
AFLP phylogenetic classification using 20 primer pairs revealed the presence of three distinct groups, with strains from Jeolla province consistently forming a separate group at a high frequency. Furthermore, M. fructicola was divided into three groups by the
AFLP pattern. Principal coordinate analysis and PERMANOVA were applied to compare strain information, such as origin, host, and fungicide sensitivity, revealing significant partition patterns for
AFLP according to geographic origin and host plants. This study represents the utilization of
AFLP methodology to investigate the genetic variability among M. fructicol isolates, highlighting the importance of continuous monitoring and management of variations in the brown rot pathogen.