Calotropisgigantea精油用于过时的药物,治疗学,和化妆品行业。然而,极端的波动性,氧化敏感性,这种油的不稳定性限制了它的应用。因此,封装是一种更有效的方法,从不利的情况下屏蔽这种油。基于Calotropisgigantea精油(CEO)的油/水(O/W)纳米乳液的创建,被称为CNE(Calotropisgigantea精油纳米乳液),对其生物潜力的评估是这项工作的目标。UV,荧光,和FT-IR方法用于生理表征。生物活动,包括消炎药,抗糖尿病,和抗癌作用。进行了CNE的药代动力学研究。发现CNE的包封效率为92%。CNE纳米乳液为球形,多分散指数为0.531,尺寸为200nm,和-35.9mV的ζ电位。即使在各种温度下储存了50天,CNE纳米乳液保持稳定。许多测试被用来确定CNE的抗氧化能力,并发现以下IC50值(µl/mL):铁螯合测定:18,羟自由基清除:37,一氧化氮自由基清除活性:58。在1μl的较高剂量下,用CNE处理后保持存活的HeLa细胞的百分比为41%。CNE以剂量依赖的方式抑制α-淀粉酶,在250µL的较高剂量下具有72%的抑制作用。对药物动力学的研究表明,纳米乳液呈现Higuchi图案。这项研究表明,Calotropisgigantea油基纳米乳液在食品中的潜在用途,化妆品,和制药行业。
Calotropis gigantea essential oil is utilized in outmoded medicine, therapeutics, and the cosmetic industries. However, the extreme volatility, oxidation susceptibility, and instability of this oil restricts its application. Thus, encapsulation is a more effective method of shielding this oil from unfavorable circumstances. The creation of oil/water (O/W)
nanoemulsions based on Calotropis gigantea essential oil (CEO), known as CNE (Calotropis gigantea essential oil
nanoemulsions), and an assessment of its biological potential were the goals of this work. UV, fluorescence, and FT-IR methods were used for physiological characterization. Biological activities, including anti-inflammatory, anti-diabetic, and anti-cancer effects. Studies on the pharmacokinetics of CNE were conducted. CNEs encapsulation efficiency was found to be 92%. The CNE
nanoemulsions had a spherical shape with polydispersity index of 0.531, size of 200 nm, and a zeta potential of -35.9 mV. Even after being stored at various temperatures for 50 days, CNE
nanoemulsions remained stable. Numerous tests were used to determine the antioxidant capacity of CNE, and the following IC50 values (µl/mL) were found: iron chelating assay: 18, hydroxyl radical scavenging: 37, and nitric oxide radical scavenging activity: 58. The percentage of HeLa cells that remained viable after being treated with CNE was 41% at a higher dose of 1 µl. CNE inhibited α-amylase in a dose-dependent manner, with 72% inhibition at its higher dose of 250 µL. Research on the kinetics of drugs showed that
nanoemulsions showed Higuchi pattern. This research showed potential use of Calotropis gigantea oil-based nanoemulsions in the food, cosmetic, and pharmaceutical industries.