Abstract：
Nanoemulsions are a promising alternative for essential oil incorporation into active coatings. The influence of the preparation steps order on nanoemulsions\' physical properties is still little explored. This study aimed to analyze the effect of the sequence of preparation steps and of the oil and polymer concentration on the stability, physical properties, and antifungal activity of alginate-based cinnamon essential oil nanoemulsions. The nanoemulsions were produced by two strategies: (I) preparation directly into an alginate solution (Ultra-Turrax at 10,000 rpm for 5 min + Ultrasound 150 W for 3 min); and (II) preparation in water (Ultra-Turrax at 10,000 rpm for 5 min + Ultrasound 150 W for 3 min) followed by homogenization with a sodium alginate solution (Ultra-Turrax at 10,000 rpm for 1, 3 or 5 min). The nanoemulsion prepared by the second strategy showed better stability, physical properties, and antifungal activity. In general, the presence of alginate hindered the cavitation effects of ultrasound, leading to the increase of droplets size and consequently affecting emulsions stability, turbidity, and antifungal properties.
摘要：
纳米乳液是将精油掺入活性涂层中的有前途的替代品。制备步骤顺序对纳米乳液物理性质的影响仍然很少探索。本研究旨在分析制备步骤的顺序以及油和聚合物浓度对稳定性的影响，物理性质，基于海藻酸盐的肉桂精油纳米乳液的抗真菌活性。通过两种策略制备纳米乳液:(I)直接制备到藻酸盐溶液中(Ultra-Turrax以10,000rpm持续5分钟+超声150W持续3分钟);和(II)在水中制备(Ultra-Turrax以10,000rpm持续5分钟+超声150W持续3分钟),然后用藻酸钠溶液均化(Ultra-Turrax以10,000rpm持续1、3或5分钟)第二种策略制备的纳米乳液显示出更好的稳定性,物理性质，和抗真菌活性。总的来说,藻酸盐的存在阻碍了超声的空化效应，导致液滴尺寸增加，从而影响乳液稳定性，浊度,和抗真菌特性。
