Abstract：
Octacosanol has various biological effects such as antioxidant, hypolipidemic and anti-fatigue. However, poor solubility has limited the application of octacosanol in food. The aim of this study was to prepare octacosanol nanoemulsions with better solubility, stability and safety and to investigate in vivo anti-fatigue effect. The food-grade formulation of the octacosanol nanoemulsions consisted of octacosanol, olive oil, Tween 80, glycerol and water with 0.1 %, 1.67 %, 23.75 %, 7.92 % and 66.65 % (w/w), respectively. The nanoemulsions had an average particle size of 12.26 ± 0.76 nm and polydispersity index of 0.164 ± 0.12, and showed good stability under different pH, cold, heat, ionic stress and long-term storage conditions. The results of animal experiments showed that the octacosanol nanoemulsions significantly prolonged the fatigue tolerance time, alleviated the fatigue-related biochemical indicators, and weakened the oxidative stress. Meanwhile, octacosanol nanoemulsions upregulated hepatic glycogen levels. Taken together, these findings suggested that octacosanol nanoemulsions have promising applications as anti-fatigue functional foods.
摘要：
二十八烷醇具有多种生物效应，如抗氧化剂，降血脂和抗疲劳。然而,溶解性差限制了二十八烷醇在食品中的应用。本研究的目的是制备溶解度较好的二十八烷醇纳米乳液,稳定性和安全性,并考察其在体内的抗疲劳作用。二十八烷醇纳米乳液的食品级配方由二十八烷醇组成，橄榄油,吐温80，甘油和含0.1%的水，1.67%，23.75%,7.92%和66.65%（w/w），分别。纳米乳液的平均粒径为12.26±0.76nm，多分散指数为0.164±0.12，在不同pH下表现出良好的稳定性。冷,热,离子胁迫和长期储存条件。动物实验结果表明,二十八烷醇纳米乳显著延长了疲劳耐受时间,缓解了疲劳相关的生化指标，并削弱了氧化应激。同时,二十八烷醇纳米乳剂上调肝糖原水平。一起来看,这些发现表明，二十八烷醇纳米乳液作为抗疲劳功能食品具有广阔的应用前景。
