Abstract：
Dunaliella salina is a promising source of β-carotene, widely employed in the food industry. This study aimed to evaluate the sequential application of the Ionic Liquid (IL) cholinium oleate as an extraction solvent for D. salina β-carotene recovery and, sequentially, as emulsifier for emulsion-based products obtained therefrom. The IL was evaluated regarding its ability to permeabilize the cells and recover β-carotene at different temperatures (25-65 °C) and IL concentrations (0-46%). The use of the IL as solvent greatly improved β-carotene recovery (>84%). The IL already present in the obtained extracts loaded with recovered β-carotene was sequentially used as emulsifier in the production of nanoemulsions (NE). NE presented a β-carotene entrapment efficiency of 100% and were kinetically stable for 30 days and presented droplet size, size distribution, and ζ-potential of 220 nm, 0.21, and -67 mV, respectively. These results indicate that using IL sequential as solvent and emulsifier has potential applications in the food industry.
摘要：
盐藻是β-胡萝卜素的一个有前途的来源，在食品工业中广泛使用。本研究旨在评估离子液体（IL）油酸胆碱作为D.salinaβ-胡萝卜素回收的提取溶剂的顺序应用，顺序，作为由其获得的乳液基产品的乳化剂。评估IL在不同温度（25-65°C）和IL浓度（0-46％）下透化细胞和回收β-胡萝卜素的能力。使用IL作为溶剂大大提高了β-胡萝卜素的回收率(>84%)。将已经存在于负载有回收的β-胡萝卜素的所得提取物中的IL依次用作纳米乳液(NE)生产中的乳化剂。NE表现出100%的β-胡萝卜素截留效率，在30天内是动力学稳定的，并且表现出液滴大小。大小分布，ζ电位为220nm，0.21和-67mV，分别。这些结果表明，使用IL序列作为溶剂和乳化剂在食品工业中具有潜在的应用。
