肝纤维化是以肝脏中瘢痕组织过度积聚为特征的病症。这种疤痕是由慢性肝损伤引起的,通常由肝炎等疾病引起,酗酒,某些代谢紊乱,遗传异常,自身免疫,和非感染性疾病,如脂肪肝,导致肝纤维化。近年来,纳米颗粒作为肝纤维化的潜在治疗剂受到关注。他们提供独特的优势,由于其体积小,大表面积,以及携带药物或靶向特定细胞或组织的能力。研究表明,纳米乳液可以增强药物递送系统,使靶向药物递送到肝脏的特定部位并改善治疗结果。在这项研究中,我们通过对使用过的农业废物脱咖啡因的棕榈枣种子进行植物化学分析来探索保护和治疗价值(PhoenixdactyliferaL.,PSC)咖啡和含咖啡因的阿拉伯咖啡种子(阿拉伯咖啡;ACS)。使用油酸/吐温80系统将ACS和PSC提取物转化为纳米乳液(NE)形式,其被招募用于治疗肝纤维化大鼠模型。透射电子显微镜(TEM)和动态光散射(DLS)用于记录尺寸,形态学,流体动力学直径,制备的NE-ACSE和NE-PSCE的ζ电位。因此,通过TEM成像的NE-ACSE和NE-PSCE,它们的ζ电位记录在20.7、23.3nm和-41.4、-28.0mV,分别。用DPPH清除测定法测定抗氧化性质。用合成的NE-PSCE和NE-ACSE治疗CCl4诱导的肝纤维化大鼠模型,通过记录炎症参数来评估每种乳液提取物在肝纤维化治疗中的作用,肝功能,抗氧化酶,和组织病理学分析结果。ACSE和PSCE的纳米乳液形式提供了抗氧化酶的显着增加,降低炎症参数,与其他群体相比,肝功能下降,与对照组接近。总之,两种纳米乳液,ACSE和PSCE,为肝脏疾病的治疗提供了新的途径,和进一步的研究被鼓励通过两种提取物的组合获得最大的治疗效率。
Liver fibrosis is a condition characterized by the excessive buildup of scar tissue in the liver. This scarring occurs as a result of chronic liver damage, often caused by conditions such as hepatitis, alcohol abuse, certain metabolic disorders, genetic abnormalities, autoimmunity, and noninfectious diseases such as fatty liver which leads to liver fibrosis. Nanoparticles have gained attention in recent years as potential therapeutic agents for liver fibrosis. They offer unique advantages due to their small size, large surface area, and ability to carry drugs or target specific cells or tissues. Studies have suggested that
nanoemulsions may enhance drug delivery systems, enabling targeted drug delivery to specific sites in the liver and improving therapeutic outcomes. In this study, we explore the protective and therapeutic values with phytochemical profiling of the used agro-wastes decaffeinated palm date seeds (Phoenix dactylifera L., PSC) coffee and caffeinated Arabic coffee seeds (Coffea arabica L.; ACS). Both ACS and PSC extracts were converted into nanoemulsion (NE) forms using the oleic acid/Tween 80 system, which was recruited for the purpose of treating a rat model with liver fibrosis. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) were used to record the sizes, morphologies, hydrodynamic diameters, and ζ-potentials of the prepared NE-ACSE and NE-PSCE. Accordingly, the NE-ACSE and NE-PSCE imaged via TEM and their ζ-potentials were recorded at 20.7, 23.3 nm and -41.4, -28.0 mV, respectively. The antioxidant properties were determined with a DPPH scavenging assay. The synthesized NE-PSCE and NE-ACSE were employed to treat a rat model with CCl4-induced liver fibrosis, to estimate the role of each emulsion-based extract in the treatment of liver fibrosis through recording inflammatory parameters, liver functions, antioxidant enzymes, and histopathological analysis results. The nanoemulsion forms of both ACSE and PSCE provided significant increases in antioxidant enzymes, reducing inflammatory parameters, compared to other groups, where liver functions were decreased with values close to those of the control group. In conclusion, both
nanoemulsions, ACSE and PSCE, provided a new avenue as therapeutic approaches for liver diseases, and further studies are encouraged to obtain maximum efficiency of treatment via the combination of both extracts.