葡萄卷叶病(GLD)是一种影响葡萄(葡萄)的病毒性疾病,对葡萄栽培具有严重的经济影响。在这项研究中,在品种cv的克隆中研究了葡萄卷叶相关病毒(GLRaV)对浆果品质的影响。红斑无核食用葡萄感染了GLRaV。RT-PCR证实了克隆的身份:克隆3236,仅用GLRaV-3感染(称为Single);克隆3215,感染了GLRaV-3,GLRaV-4毒株9和葡萄病毒A(称为混合),和与感染克隆具有相同遗传背景的无病毒克隆(称为对照)。浆果的大小质量指标,糖,酸度,在收获成熟时测量花青素含量。RT-qPCR用于确定病毒载量。这项研究重复了两年。双向,应用多变量方差分析(MANOVA),以克隆和年份为自变量,以测量的浆果品质参数为因变量。所有因变量都受到病毒感染的显著影响(威尔克斯,λ,[2,33]=0.033895,p值<0.001),而只有可滴定酸度(TA)受到年份的影响。平均浆果干重下降(p值<0.001)。两个感染克隆的水含量均大于对照(p值<0.001)。两个受感染的克隆显示作为浆果干重的一部分的糖含量降低(p值<0.001)。与对照克隆相比,感染克隆的花色苷和苯酚含量显着降低(分别为P<0.001,P<0.05,克隆3236和克隆3215)。最后,病毒载量变化很大,病毒载量与浆果成分之间没有定量关系。
Grapevine leafroll disease is a viral disease that affects grapevines (Vitis vinifera L.) and has a severe economic impact on viticulture. In this study, the effect of grapevine leafroll-associated viruses (GLRaV) on berry quality was investigated in clones of cultivar cv. Crimson Seedless table grapes infected with GLRaV. RT-PCR confirmed the identity of the clones:
clone 3236, infected only with GLRaV-3 (termed single);
clone 3215, infected with GLRaV-3, GLRaV-4 strain 9 and grapevine virus A (termed mixed); and a viral free
clone of the same genetic background of the infected clones (termed control). The berry quality indices of size, sugar, acidity and anthocyanin content were measured at harvest maturity. RT-qPCR was used to determine the viral load. The study was repeated over 2 year. A two-way, multivariate analysis of variance was applied with
clone and year as independent variables and the measured berry quality parameters as a dependent variable. All dependent variables were significantly affected by viral infection (Wilks, λ, (2,33) = 0.033895, P-value <0.001), while only titratable acidity was affected by year. The average berry dry mass decreased (P-value <0.001). The water content of both infected clones was greater than that of the control (P-value <0.001). Both infected clones displayed reduced sugar content as a fraction of the berry dry mass (P-value <0.001). The anthocyanin and the phenol content of the infected clones were significantly reduced compared with the control clone (P < 0.001, P < 0.05,
clone 3236 and
clone 3215, respectively). Finally, the viral load was highly variable, and no quantitative relationship between viral load and berry composition was found.