Abstract：
Cell line panels have proven to be an invaluable tool for investigators researching a range of topics from drug mechanism or drug sensitivity studies to disease-specific etiology. The cell lines used in these panels may range from heterogeneous tumor populations grown from primary tumor isolations to genetically engineered clonal cell lines which express specific gene isoforms. Mouse embryonic fibroblast (MEF) cells are a commonly used cell line for biological research due to their accessibility and ease of genetic manipulation. This chapter will describe the process of creating a size-sorted diploid (SSDC) clonal cell panel expressing specific RAS isoforms from a previously engineered RAS-less MEF cell line pool.
摘要：
细胞系小组已被证明是研究人员从药物机制或药物敏感性研究到疾病特异性病因的一系列主题的宝贵工具。这些组中使用的细胞系的范围可以是从原代肿瘤分离生长的异质肿瘤群体到表达特定基因同种型的基因工程克隆细胞系。小鼠胚胎成纤维细胞(MEF)细胞是用于生物学研究的常用细胞系,因为它们的可接近性和易于进行遗传操作。本章将描述从先前工程化的无RASMEF细胞系池创建表达特定RAS同工型的大小分选的二倍体（SSDC）克隆细胞组的过程。
