Abstract：
The gene encoding juvenile hormone response (Krüppel homolog1, Kr-hl) in Coccinella septempunctata was investigated by cloning and analysing expression profiles in different developmental stages and tissues by quantitative real-time polymerase chain reaction (PCR). C. septempunctata Kr-hl (CsKr-hl) encoded a 1338 bp open reading frame (ORF) with a predicted protein product of 445 amino acids; the latter showed high similarity to orthologs in other species and contained eight highly-conserved Zn-finger motifs for DNA-binding. CsKr-hl was expressed in different developmental stages of C. septempunctata. The expression levels of CsKr-hl in eggs, 2nd, 3rd, 4th instar larvae, and pupa were 3.31, 2.30, 7.09, 0.58, and 7.48 times the number of 1st instar larvae, respectively. CsKr-hl expression levels in female adults gradually increased at 25-30 days and were significantly higher than expression at 1-20 days. CsKr-hl expression in 20-30 days-old male adults was significantly higher than males aged 1-15 days. CsKr-hl expression levels in heads of male and female adults were significantly higher than expression levels in the thorax, adipose, and reproductive system. Interestingly, CsKr-hl expression levels in the adipose and reproductive system of female adults were significantly higher than in adult male corresponding organs, which suggest that CsKr-hl plays an important role in regulating reproductive development in C. septempunctata.
摘要：
通过定量实时聚合酶链反应（PCR）克隆和分析不同发育阶段和组织中的表达谱，研究了七球菌中编码少年激素反应的基因（Krüppel同源物1，Kr-hl）。C.septempunctataKr-hl（CsKr-hl）编码一个1338bp的开放阅读框（ORF），其预测的蛋白质产物为445个氨基酸；后者与其他物种的直向同源物表现出高度相似性，并包含八个高度保守的Zn指基序用于DNA结合。CsKr-hl在C.septempunctata的不同发育阶段表达。鸡蛋中CsKr-hl的表达水平,2nd,3rd,4龄幼虫,蛹分别是一龄幼虫数量的3.31、2.30、7.09、0.58和7.48倍，分别。CsKr-hl在成年女性中的表达水平在25-30天逐渐增加,并且显著高于在1-20天的表达。CsKr-hl在20-30日龄男性成人中的表达显著高于1-15日龄男性。CsKr-hl在男性和女性成虫头部的表达水平显著高于胸部的表达水平,脂肪,和生殖系统。有趣的是,CsKr-hl在成年女性脂肪和生殖系统中的表达水平明显高于成年男性相应器官,这表明CsKr-hl在C.septempunctata的生殖发育中起重要作用。
