PDF(Pubmed)
Abstract：
The potential role of the juvenile hormone receptor gene (methoprene-tolerant, Met) in reproduction of Coccinella septempunctata L. (Coleoptera: Coccinellidae)(Coleoptera: Coccinellidae), was investigated by cloning, analyzing expression profiles by quantitative real-time PCR, and via RNA interference (RNAi). CsMet encoded a 1518-bp open reading frames with a predicted protein product of 505 amino acids; the latter contained 2 Per-Arnt-Sim repeat profile at amino acid residues 30-83 and 102-175. CsMet was expressed in different C. septempunctata larvae developmental stages and was most highly expressed in third instar. CsMet expression in female adults gradually increased from 20 to 30 d, and expression levels at 25 and 30 d were significantly higher than levels at 1-15 d. CsMet expression in 20-d-old male adults was significantly higher than in males aged 1-15 d. CsMet expression levels in fat body tissues of male and female adults were significantly higher than expression in the head, thorax, and reproductive system. At 5 and 10 d after CsMet-dsRNA injection, CsMet expression was significantly lower than the controls by 75.05% and 58.38%, respectively. Ovary development and vitellogenesis in C. septempunctata injected with CsMet-dsRNA were significantly delayed and fewer mature eggs were produced. This study provides valuable information for the large-scale rearing of C. septempunctata.
摘要：
幼体激素受体基因的潜在作用（耐甲氧烯，Met）在七叶球藻（鞘翅目：球藻）（鞘翅目：球藻）的繁殖中，通过克隆进行了调查，通过定量实时PCR分析表达谱，并通过RNA干扰(RNAi)。CsMet编码1518bp的开放阅读框，预测的蛋白质产物为505个氨基酸；后者在氨基酸残基30-83和102-175处包含2个Per-Arnt-Sim重复序列。CsMet在不同的C.septempintunctata幼虫发育阶段表达，在三龄中表达最高。CsMet在成年女性中的表达从20到30d逐渐增加，25和30d时的表达水平显着高于1-15d时的水平。CsMet在20d男性成年人中的表达显着高于1-15d男性。CsMet在男性和女性成年人的脂肪体组织中的表达水平显着高于头部中的表达。胸部,和生殖系统。CsMet-dsRNA注射后5天和10天，CsMet表达比对照组显著降低了75.05%和58.38%,分别。注射CsMet-dsRNA的C.septempunctata中的卵巢发育和卵黄发生显着延迟，并且产生了较少的成熟卵。本研究为七叶草的大规模饲养提供了有价值的信息。
