PDF(Pubmed)
Abstract：
The receptor for activated protein kinase C1 (RACK1) belongs to the typical WD repeat family, which is extremely conservative and important in multiple signal transduction pathways related to growth and development that coordinate the intracellular role of various life activities. As a novel protein with versatile functions, it was found in a variety of organisms. In a previous study, we identified the RACK1 sequence of white shrimp from transcriptome data. In this study, we employed specialized bioinformatics software to conduct an in-depth analysis of EcRACK1 and compare its amino acid sequence homology with other crustaceans. Furthermore, we investigated the expression patterns of RACK1 at different developmental stages and tissues, as well as at various time points after exposure to Aroclor 1245, aiming to elucidate its function and potential response towards Aroclor 1245 exposure. The length of EcRACK1 is 957 nucleotides, which encodes 318 amino acids. Moreover, there were seven typical WD repeats in EcRACK1, which have more than a 96% sequence identity with the RACK1 proteins of Penaeus. The results of tissue expression and spatiotemporal expression showed that it was significantly increased in the II and IV stages, but had a significant tissue specificity in the hepatopancreas, spermary, and muscle tissues of E. carinicauda, adult stage. Compared to the control, EcRACK1 was significantly induced in E. carinicauda zoea larvae exposed to Aroclor 1254 for 6, 10, 20, and 30 d (p < 0.05). These results suggested that EcRACK1 may play an important role in the larval development and environmental defense of E. carinicauda.
摘要：
活化蛋白激酶C1受体(RACK1)属于典型的WD重复家族,在与生长发育相关的多条信号转导通路中，协调各种生命活动的细胞内作用，是极其保守和重要的。作为一种具有多种功能的新型蛋白质，它被发现在各种生物中。在之前的研究中,我们从转录组数据中鉴定了白虾的RACK1序列。在这项研究中,我们使用专门的生物信息学软件对EcRACK1进行了深入分析,并比较了其与其他甲壳类动物的氨基酸序列同源性.此外，我们研究了RACK1在不同发育阶段和组织中的表达模式，以及暴露于Aroclor1245后的各个时间点，旨在阐明其功能和对Aroclor1245暴露的潜在反应。EcRACK1的长度为957个核苷酸，编码318个氨基酸。此外，EcRACK1中有7个典型的WD重复序列，与对虾的RACK1蛋白具有96％以上的序列同一性。组织表达和时空表达的结果表明，在II期和IV期明显升高，但在肝胰腺中有明显的组织特异性，Spermary,和Carinicauda的肌肉组织，成人阶段。与对照相比，在暴露于Aroclor12546、10、20和30d的Carinicauda幼虫中，EcRACK1被显着诱导（p<0.05）。这些结果表明，EcRACK1可能在Carinicauda的幼虫发育和环境防御中起重要作用。
