背景:斑马鱼是脊椎动物,在中枢神经系统损伤后具有很高的再生潜力。因此,它们已成为研究创伤性脊髓损伤的有用模型系统。
方法:使用幼体斑马鱼,我们开发了一个强大的范例来模拟前脊髓损伤的影响,这对应于人类颈部和胸部的衰弱损伤。与以前的研究相比,我们的新范式包括更前部的损伤位置,使用视觉运动反应的改良行为评估,和一个新的数据分析代码。
结果:我们的方法使脊髓损伤更接近后脑,与以前使用更后部损伤位置的研究相比,功能影响更大。这项工作报告的结果揭示了脊髓损伤后七天内的恢复。
■本工作描述了使用幼体斑马鱼进行损伤后脊髓再生的体内研究的改良范例,包括前部损伤位置,一个强有力的行为评估,和一个新的数据分析软件。
结论:我们的发现为应用这一范式研究药物的作用奠定了基础。营养,和其他处理,以改善再生过程。
BACKGROUND: Zebrafish are vertebrates with a high potential of regeneration after injury in the central nervous system. Therefore, they have emerged as a useful model system for studying traumatic spinal cord injuries.
METHODS: Using larval zebrafish, we have developed a robust paradigm to model the effects of anterior spinal cord injury, which correspond to the debilitating injuries of the cervical and thoracic regions in humans. Our new paradigm consists of a more anterior injury location compared to previous studies, a modified behavioral assessment using the visual motor response, and a new data analysis code.
RESULTS: Our approach enables a spinal cord injury closer to the hindbrain with more functional impact compared to previous studies using a more posterior injury location. Results reported in this work reveal recovery over seven days following spinal cord injury.
UNASSIGNED: The present work describes a modified paradigm for the in vivo study of spinal cord regeneration after injury using larval zebrafish, including an anterior injury location, a robust behavioral assessment, and a new data analysis software.
CONCLUSIONS: Our findings lay the foundation for applying this paradigm to study the effects of drugs, nutrition, and other treatments to improve the regeneration process.