随着适当的微环境恢复丢失的生物电信号传输是脊髓再生的主要临床挑战之一。在目前的研究中,我们开发了基于多糖的蛋白质复合材料多壁碳纳米管(MWCNTs)/胶原蛋白(Col)/透明质酸(HA)与橙皮苷(Hes)天然化合物的复合材料,以研究其联合治疗效果以及生物相容性。抗氧化活性,和导电性。通过FT-IR对多功能复合材料进行了表征,XRD,SEM,HR-TEM,BET,C.V,和EIS技术。MWCNT-Col-HA-Hes的电导率和模量分别为0.06S/cm和12.3kPa,类似于天然脊髓。体外细胞毒性,细胞活力,抗氧化性能,用PC-12细胞系研究了制备的复合材料的细胞迁移能力。体外研究表明,多功能复合材料显示出更高的细胞活力,抗氧化剂,和细胞迁移特性比对照细胞。ROS水平的降低表明复合物中Hes的存在可以通过保护其免受氧化损伤和促进细胞向损伤部位迁移来降低细胞应激。开发的多功能复合材料可以提供具有相容性的抗氧化微环境,并通过为脊髓组织再生提供适当的电导率和机械强度来模拟天然脊髓。
Restoring the lost bioelectrical signal transmission along with the appropriate microenvironment is one of the major clinical challenges in spinal cord regeneration. In the current research, we developed a polysaccharide-based protein composite Multiwalled Carbon Nanotubes (MWCNTs)/ Collagen (Col)/ Hyaluronic acid (HA) composite with Hesperidin (Hes) natural compound to investigate its combined therapeutic effect along with biocompatibility, antioxidant activity, and electrical conductivity. The multifunctional composites were characterized via FT-IR, XRD, SEM, HR-TEM, BET, C.V, and EIS techniques. The electrical conductivity and modulus of the MWCNT-Col-HA-Hes were 0.06 S/cm and 12.3 kPa, similar to the native spinal cord. The in-vitro Cytotoxicity, cell viability, antioxidant property, and cell migration ability of the prepared composites were investigated with a PC-12 cell line. In-vitro studies revealed that the multifunctional composites show higher cell viability, antioxidant, and cell migration properties than the control cells. Reduction of ROS level indicates that the Hes presence in the composite could reduce the cell stress by protecting it from oxidative damage and promoting cell migration towards the lesion site. The developed multifunctional composite can provide the antioxidant microenvironment with compatibility and mimic the native spinal cord by providing appropriate conductivity and mechanical strength for spinal cord tissue regeneration.