背景:最近,关注肠道微生物组和肥胖或炎性疾病之间关联的研究数量有所增加,尤其是成年人。然而,缺乏调查青少年肠道微生物组和胃肠道(GI)疾病之间关联的研究.
方法:我们从202名青少年获得了16SrRNA-seq数据集,用于肠道微生物组分析,包括溃疡性结肠炎(UC),克罗恩病(CD),肥胖(Ob),和健康对照(HC)。我们利用对微生物生态学的定量见解(QIIME)和通过重建不受保护的国家(PICRUSt)对社区进行系统发育研究,以获取操作分类单位(OTU)。随后,我们分析了京都基因和基因组百科全书(KEGG)体形学(KO)术语和途径富集鉴定的OTU。
结果:在这项研究中,我们使用202份16SrRNA测序数据,调查了胃肠道疾病青少年和健康青少年肠道微生物组之间的差异.六种主要肠道微生物群的分布(即,未分类的Dorea,未分类的仙人掌科,未分类的Ruminococus,普氏粪杆菌,CopriPrevotella,未分类的Sutterilla)根据肥胖和炎症性疾病的状况而有所不同。在患有炎症性疾病的青少年中,在Lachnospirosaceae中观察到菌群失调(即,UC和CD),以及普雷沃氏菌和梭菌中肥胖的青少年。更具体地说,我们的结果表明,普氏粪杆菌和未分类的蛇床子科的相对丰度分别高于10%和8%,分别,在UC组中,与CD相比,Ob,HC组。此外,Ob组有超过20%和超过3%的高水平的Prevotellacombri和未分类的Sutterella,分别,与UC相比,CD,HC组。此外,检查六个特定微生物群和KO术语之间的关联,我们发现6个与微生物群相关的KO术语与NOD样受体信号相关.这六个分类群差异可能通过影响关键青春期宿主中的NOD样受体信号传导来影响免疫系统和炎症反应。
结论:在这项研究中,我们发现,在患有炎症性疾病和肥胖的青少年中,微生物群落的菌群失调对炎症和免疫应答途径有不同程度的影响。
BACKGROUND: Recently, there has been an increase in the number of studies focusing on the association between the gut microbiome and obesity or inflammatory diseases, especially in adults. However, there is a lack of studies investigating the association between gut microbiome and gastrointestinal (GI) diseases in adolescents.
METHODS: We obtained 16S rRNA-seq datasets for gut microbiome analysis from 202 adolescents, comprising ulcerative colitis (UC), Crohn\'s disease (CD), obesity (Ob), and healthy controls (HC). We utilized Quantitative Insights Into Microbial Ecology (QIIME) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) to acquire Operational Taxonomic Units (OTUs). Subsequently, we analyzed Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology (KO) terms and pathway enrichment for the identified OTUs.
RESULTS: In this study, we investigated the difference between the gut microbiomes in adolescents with GI diseases and those in healthy adolescents using 202 samples of 16S rRNA sequencing data. The distribution of the six main gut microbiota (i.e., unclassified Dorea, unclassified Lachnospiraceae, unclassified Ruminococcus, Faecalibacterium prausnitzii,
Prevotella copri, unclassified Sutterella) was different based on the status of obesity and inflammatory diseases. Dysbiosis was observed within Lachnospiraceae in adolescents with inflammatory diseases (i.e., UC and CD), and in adolescents with obesity within
Prevotella and Sutterella. More specifically, our results showed that the relative abundance of Faecalibacterium prausnitzii and unclassified Lachnospiraceae was more than 10% and 8% higher, respectively, in the UC group compared to the CD, Ob, and HC groups. Additionally, the Ob group had over 20% and over 3% higher levels of
Prevotella copri and unclassified Sutterella, respectively, compared to the UC, CD, and HC groups. Also, inspecting associations between the six specific microbiota and KO terms, we found that the six microbiota -relating KO terms were associated with NOD-like receptor signaling. These six taxa differences may affect the immune system and inflammatory response by affecting NOD-like receptor signaling in the host during critical adolescence.
CONCLUSIONS: In this study, we discovered that dysbiosis of the microbial community had varying degrees of influence on the inflammatory and immune response pathways in adolescents with inflammatory diseases and obesity.