PDF(Pubmed)
Abstract：
UNASSIGNED: Numerous bacteria are involved in the etiology of bacterial vaginosis (BV). Yet, current tests only focus on a select few. We therefore designed a new test targeting 22 BV-relevant species.
UNASSIGNED: Using 946 stored vaginal samples, a new qPCR test that quantitatively identifies 22 bacterial species was designed. The distribution and relative abundance of each species, α- and β-diversities, correlation, and species co-existence were determined per sample. A diagnostic index was modeled from the data, trained, and tested to classify samples into BV-positive, BV-negative, or transitional BV.
UNASSIGNED: The qPCR test identified all 22 targeted species with 95 - 100% sensitivity and specificity within 8 hours (from sample reception). Across most samples, Lactobacillus iners, Lactobacillus crispatus, Lactobacillus jensenii, Gardnerella vaginalis, Fannyhessea (Atopobium) vaginae, Prevotella bivia, and Megasphaera sp. type 1 were relatively abundant. BVAB-1 was more abundant and distributed than BVAB-2 and BVAB-3. No Mycoplasma genitalium was found. The inter-sample similarity was very low, and correlations existed between key species, which were used to model, train, and test a diagnostic index: MDL-BV index. The MDL-BV index, using both species and relative abundance markers, classified samples into three vaginal microbiome states. Testing this index on our samples, 491 were BV-positive, 318 were BV-negative, and 137 were transitional BV. Although important differences in BV status were observed between different age groups, races, and pregnancy status, they were statistically insignificant.
UNASSIGNED: Using a diverse and large number of vaginal samples from different races and age groups, including pregnant women, the new qRT-PCR test and MDL-BV index efficiently diagnosed BV within 8 hours (from sample reception), using 22 BV-associated species.
UNASSIGNED: Using 946 stored vaginal samples, a new qPCR test that quantitatively identifies 22 bacterial species was designed. The distribution and relative abundance of each species, α- and β-diversities, correlation, and species co-existence were determined per sample. A diagnostic index was modeled from the data, trained, and tested to classify samples into BV-positive, BV-negative, or transitional BV.
UNASSIGNED: The qPCR test identified all 22 targeted species with 95 - 100% sensitivity and specificity within 8 hours (from sample reception). Across most samples, Lactobacillus iners, Lactobacillus crispatus, Lactobacillus jensenii, Gardnerella vaginalis, Fannyhessea (Atopobium) vaginae, Prevotella bivia, and Megasphaera sp. type 1 were relatively abundant. BVAB-1 was more abundant and distributed than BVAB-2 and BVAB-3. No Mycoplasma genitalium was found. The inter-sample similarity was very low, and correlations existed between key species, which were used to model, train, and test a diagnostic index: MDL-BV index. The MDL-BV index, using both species and relative abundance markers, classified samples into three vaginal microbiome states. Testing this index on our samples, 491 were BV-positive, 318 were BV-negative, and 137 were transitional BV. Although important differences in BV status were observed between different age groups, races, and pregnancy status, they were statistically insignificant.
UNASSIGNED: Using a diverse and large number of vaginal samples from different races and age groups, including pregnant women, the new qRT-PCR test and MDL-BV index efficiently diagnosed BV within 8 hours (from sample reception), using 22 BV-associated species.
摘要：
■许多细菌与细菌性阴道病（BV）的病因有关。然而，目前的测试只集中在少数人身上。因此，我们设计了一种针对22种BV相关物种的新测试。
■使用946个储存的阴道样本，设计了一种新的qPCR测试，定量鉴定22种细菌。每个物种的分布和相对丰度，α-和β-多样性，相关性，并确定每个样品的物种共存。根据数据建立诊断指数，受过训练，并将样本分类为BV阳性，BV-负,或过渡性BV。
■qPCR测试在8小时内（从样品接收开始）以95-100％的灵敏度和特异性鉴定了所有22种目标物种。在大多数样品中，乳酸菌，卷曲乳杆菌,詹氏乳杆菌,阴道加德纳菌,范尼海（Atobobium）阴道，比维亚普雷沃氏菌，和Megasphaerasp.1型相对丰富。BVAB-1比BVAB-2和BVAB-3更丰富和分布。未发现生殖支原体。样本间相似度很低,关键物种之间存在相关性，用来建模的，火车,并测试诊断指标：MDL-BV指数。MDL-BV指数，使用物种和相对丰度标记，将样本分为三种阴道微生物组状态。在我们的样本上测试这个指数，491例BV阳性,318是BV阴性，137是过渡性BV。尽管在不同年龄段之间观察到BV状态的重要差异，种族,和怀孕状态，它们在统计上微不足道。
■使用来自不同种族和年龄组的多样化和大量的阴道样本，包括孕妇,新的qRT-PCR测试和MDL-BV指数在8小时内有效诊断BV（从样品接收），使用22个BV相关物种。
■使用946个储存的阴道样本，设计了一种新的qPCR测试，定量鉴定22种细菌。每个物种的分布和相对丰度，α-和β-多样性，相关性，并确定每个样品的物种共存。根据数据建立诊断指数，受过训练，并将样本分类为BV阳性，BV-负,或过渡性BV。
■qPCR测试在8小时内（从样品接收开始）以95-100％的灵敏度和特异性鉴定了所有22种目标物种。在大多数样品中，乳酸菌，卷曲乳杆菌,詹氏乳杆菌,阴道加德纳菌,范尼海（Atobobium）阴道，比维亚普雷沃氏菌，和Megasphaerasp.1型相对丰富。BVAB-1比BVAB-2和BVAB-3更丰富和分布。未发现生殖支原体。样本间相似度很低,关键物种之间存在相关性，用来建模的，火车,并测试诊断指标：MDL-BV指数。MDL-BV指数，使用物种和相对丰度标记，将样本分为三种阴道微生物组状态。在我们的样本上测试这个指数，491例BV阳性,318是BV阴性，137是过渡性BV。尽管在不同年龄段之间观察到BV状态的重要差异，种族,和怀孕状态，它们在统计上微不足道。
■使用来自不同种族和年龄组的多样化和大量的阴道样本，包括孕妇,新的qRT-PCR测试和MDL-BV指数在8小时内有效诊断BV（从样品接收），使用22个BV相关物种。
