背景:单细胞RNA测序(scRNA-seq)已广泛应用于分析正常和患病皮肤的细胞异质性。皮脂腺,具有维持皮肤完整性的既定功能和在全身能量代谢中的新兴作用的基本皮肤成分,在scRNA-seq研究中被大量忽视。
方法:从小鼠和人类皮肤scRNA-seq数据集出发,我们用开源的R-packageosSOM鉴定了特别在皮脂腺中表达的基因集。
结果:鉴定的基因集包括皮脂腺典型基因,如Scd3,Mgst1,Cidea,Awat2和KRT7。令人惊讶的是,然而,最高的100人中没有一个重叠,仅在小鼠和人类样本中的皮脂腺中表达转录本。值得注意的是,这两个物种只有25个转录本,包括参与脂肪酸的线粒体和过氧化物酶体基因,氨基酸,和葡萄糖加工,从而突出了这个腺体的强烈代谢率。
结论:这项研究强调了小鼠和人类皮脂腺脂质合成的内在差异,并表明在这种情况下过氧化物酶体过程的重要作用。我们的数据还为实验解决调节皮脂腺稳态的新候选物提供了有吸引力的起点。
BACKGROUND: Single-cell RNA sequencing (scRNA-seq) has been widely applied to dissect cellular heterogeneity in normal and diseased skin. Sebaceous glands, essential skin components with established functions in maintaining skin integrity and emerging roles in systemic energy metabolism, have been largely neglected in scRNA-seq studies.
METHODS: Departing from mouse and human skin scRNA-seq datasets, we identified gene sets expressed especially in sebaceous glands with the open-source R-package oposSOM.
RESULTS: The identified gene sets included sebaceous gland-typical genes as Scd3, Mgst1, Cidea, Awat2 and KRT7. Surprisingly, however, there was not a single overlap among the 100 highest, exclusively in sebaceous glands expressed transcripts in mouse and human samples. Notably, both species share a common core of only 25 transcripts, including mitochondrial and peroxisomal genes involved in fatty acid, amino acid, and glucose processing, thus highlighting the intense metabolic rate of this gland.
CONCLUSIONS: This study highlights intrinsic differences in sebaceous lipid synthesis between mice and humans, and indicates an important role for peroxisomal processes in this context. Our data also provides attractive starting points for experimentally addressing novel candidates regulating sebaceous gland homeostasis.