PDF(Pubmed)
Abstract：
Cattle mange causes extreme itchiness, and the associated stress is an animal welfare concern that leads to economic losses due to decreased cattle productivity and deworming costs. This study investigated the reason why Chorioptic mites, C. bovis and C. texanus, preferentially infest the tail root region (rTR) and performed histological and biochemical analysis focusing on the volatile components of host odors that serve as the starting point for infestation of parasitic arthropods. Skin samples were taken from the rTR, lateral abdominal, and central masseteric, with the latter two designated as comparison sites. The two and three-dimensional histological analysis measured each sebaceous and sweat gland percentage per unit volume. The q-PCR analyzed the expression levels of ALDH1A1 and LOC785756, which are genes associated with volatile odoriferous compounds that serve as repellency and attractive messengers for ticks. Immunohistochemistry stained three sites with anti-androgen binding protein beta-like (ABPβ-like), encoded by LOC785756, antibody. The three-dimensional analysis showed that sebaceous glands in the rTR tend to be more continuous and existed in larger masses than in other regions. The expression level of LOC785756 was significantly higher in the rTR, and immunohistochemistry revealed the presence of ABPβ-like in the sebaceous gland with strong positive signals in the rTR. These results suggest that C. bovis/texanus selectively infests the rTR because that skin has well-developed sebaceous glands, including a large amount of ABPβ-like, which acts as a mite attractant.
摘要：
牛皮会导致极度瘙痒，相关的压力是动物福利问题，由于牛的生产力下降和驱虫成本而导致经济损失。这项研究调查了绒毛膜螨的原因，C.牛和C.texanus，优先侵染尾根区域（rTR），并进行组织学和生化分析，重点是宿主气味的挥发性成分，这些成分是寄生节肢动物侵染的起点。皮肤样本取自rTR,外侧腹部，和中央按摩师，后两者被指定为比较地点。二维和三维组织学分析测量每单位体积的皮脂腺和汗腺百分比。q-PCR分析了ALDH1A1和LOC785756的表达水平，它们是与挥发性气味化合物相关的基因，这些化合物可作为壁虱的驱避性和有吸引力的信使。免疫组织化学用抗雄激素结合蛋白β样（ABPβ样）染色三个部位，由LOC785756，抗体编码。三维分析表明，与其他区域相比，rTR中的皮脂腺趋于更连续，并且存在更大的质量。LOC785756在rTR中的表达程度显著增高，免疫组织化学显示皮脂腺中存在ABPβ样，rTR中存在强阳性信号。这些结果表明，C.bovis/texanus选择性地感染rTR，因为皮肤有发达的皮脂腺，包括大量ABPβ样，充当螨引诱剂。
