背景:从脂肪组织中分离的细胞外基质,称为无细胞脂肪基质(AAM),代表一种新型生物材料。AAM作为支架起作用,其不仅支持干细胞增殖和分化,而且诱导脂肪形成和血管生成。本研究旨在研究与常规脂肪移植相比,可注射AAM的体积效应和微环境变化。
方法:AAM是使用机械修饰的方法从新鲜的人腹部成形术脂肪制成的,然后转化为可注射形式。使用Coleman技术收获脂肪抽吸物。通过将可注射的AAM或脂肪抽吸物注射到头皮中对无胸腺裸鼠进行重量和体积研究(每组n=6)。八周后,使用显微计算机断层扫描(micro-CT)扫描通过重量测量和体积分析评估移植物保留.使用免疫荧光染色对perilipin和CD31进行组织学分析。
结果:可注射AAM在小鼠模型中表现出相似的重量和体积效应。组织学分析显示,与常规脂肪移植物相比,炎症细胞的存在相当,胶囊形成最少。脂肪发生在AAM注射和常规脂肪移植模型中,血管面积(%)二者之间无显著差异。
结论:总之,可注射AAM在软组织重建中的体积效应和组织再生方面证明了与常规脂肪移植相当的有效性。这种有前途的同种异体注射剂具有在美学和重建临床实践中作为安全有效的“现成”替代品的潜力。
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BACKGROUND: The extracellular matrix isolated from adipose tissue, known as acellular adipose matrix (AAM), represents a novel biomaterial. AAM functions as a scaffold that not only supports stem cell proliferation and differentiation but also induces adipogenesis and angiogenesis. This study aims to investigate the volumetric effects and microenvironmental changes associated with injectable AAM in comparison to conventional fat grafting.
METHODS: AAM was manufactured from fresh human abdominoplasty fat using a mechanically modified method and then transformed into an injectable form. Lipoaspirate was harvested employing the Coleman technique. A weight and volume study was conducted on athymic nude mice by injecting either injectable AAM or lipoaspirate into the scalp (n=6 per group). After eight weeks, graft retention was assessed through weight measurement and volumetric analysis using micro-computed tomography (micro-CT) scanning. Histological analysis was performed using immunofluorescence staining for perilipin and CD31.
RESULTS: Injectable AAM exhibited similar weight and volume effects in murine models. Histological analysis revealed comparable inflammatory cell presence with minimal capsule formation when compared to conventional fat grafts. Adipogenesis occurred in both AAM-injected and conventional fat graft models, with no significant difference in the blood vessel area (%) between the two.
CONCLUSIONS: In summary, injectable AAM demonstrates effectiveness comparable to conventional fat grafting concerning volume effects and tissue regeneration in soft tissue reconstruction. This promising allogeneic injectable holds the potential to serve as a safe and effective \"Off-the-Shelf\" alternative in both aesthetic and reconstructive clinical practices.
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